Neutral red

A. squamosa was purchased from Guangzhou Tianhe fruit wholesale market, Guangzhou, China. The pulp material was identified by Professor R.M. Yu, College of Pharmacy, Jinan University, China. Standard monosaccharides and T-series dextrans were obtained from Sigma Chemical Co. (St. Louis, MO, USA). DEAE-52 cellulose and Sephadex G-100 were obtained from Whatman Ltd (Kent County, England). Sephacryl S-300 HR was obtained from Amersham Biosciences (Pharmacia, Uppsala, Sweden). The rmGM-CSF (214-14) and rmIL-4 (315-03) were obtained from PeproTechInc (Rocky Hill, NJ, USA). Anti-CD11c-FITC, anti-MHC II-PE, anti-CD86-FITC and anti-CD11c-APC antibodies were purchased from eBioscience (San Diego, CA, USA). Neutral red was purchased from Amresco (Albany, NY, USA) and the NO assay kit was supplied by the Beyotime Institute of Biotechnology (Haimen, China). Lipopolysaccharide (LPS) and FITC-dextran were purchased from Sigma–Aldrich (St. Louis, MO, USA). Anti-β-actin and anti-GAPDH antibodies were obtained from Biosharp (Beijing, China). Anti-Histone-H3 antibody was obtained from Proteintech (Wuhan, China). MAPK family antibody sampler kit and NF-κB pathway sampler kit were purchased from Cell Signaling Technology (Beverly, MA, USA). Other chemicals and reagents were of analytical grade.