Sis megaview ssd digital camera

In the present study, TEM was used in order to measure the thickness of the glomerular basal membrane and distinguish class 0 from class I DKD. Small specimens of renal tissue were retrieved from paraffin blocks, de-paraffined in xylene, rehydrated in ethanol (100%, 95% and 70%) and washed in 0.15 M sodium cacodylate buffer. After post-fixation in 1% osmium tetroxide, the samples were washed with increasing concentration of ethanol (from 70 % to 100%), embedded in Araldite resin and cut with the ultramicrotome. Ultrathin sections were stained with uranyl acetate and lead citrate before the examination with Philips CM10 (FEI Company, Milan, Italy) Transmission Electron Microscope equipped with a Gatan camera. For each sample, five digital images were randomly acquired using the FEI proprietary software Olympus SIS Megaview SSD digital camera. The thickness of the Glomerular Basement Membrane (GBM) was measured in twelve different positions at 13500 of magnification. Class I DKD was defined as the presence of basal membranes with average thickness >430 nm in male patients and >395 nm in female patients [29 (link)].