1 decyne

A mixture of n-alkanes (C₈—C₂₀) dissolved in hexane for retention index determination was purchased from Fluka (Buchs, Switzerland). Reference compounds, (E)-2-hexen-1-ol (> 96%), (E)-2-octen-1-ol (> 97%), 1-decyne (> 98%), 1-dodecene (≥ 99%), (E,E)-2,4-hexadienal (≥ 97%), 2-cyclohexen-1-ol (> 95%), (E)-2-hexenal (≥ 99%), (E)-2-octenal (≥ 95%), 4-tert-butylcyclohexyl acetate (mixture of E and Z, > 98%), allyl cyclohexanepropionate (≥ 98%), cyclopentanemethanol (≥ 98%), cyclopentanol (≥ 99%), cyclopentanone (≥ 99%), decanal (≥ 98%), 1-dodecanol (≥ 98%), dodecyl acetate (> 97%), hexyl acetate (≥ 99%), hexyl salicylate (≥ 99%), limonene (> 97%), nonanal (≥ 97%), octyl acetate (≥ 99%), p-cymene (> 99%), 1-tridecanol (≥ 99%), and α-hexylcinnamaldehyde (≥ 95%) were supplied by Sigma Aldrich (Munich, Germany).

A mixture of n-alkanes (C6–C10) dissolved in pentane was purchased from Fluka (Buchs, Switzerland) and α-pinene (≥95%) from Merck. All other reference compounds were supplied by Sigma-Aldrich (Munich, Germany): (E)-2-hexen-1-ol (≥ 95%), (E)-2-octenal (≥94%), (E)-2-nonenal (≥93%), hexyl acetate (99%), 1-decyne (98%), (Z)-3-hexenyl acetate (≥98%), (E)-2-decenal (analytical standard), 3-methyl-1-butanol acetate (isoamyl acetate) (≥97%), benzaldehyde (≥99%), nonanal (97%), (E)-2-hexenal (98%), (E)-2-octen-1-ol (97%), decanal (≥98%), 2,4-octadienal (96%), (E,E)-2,4-decadienal (analytical standard), (R)-(+)-limonene (97%), (E,E)-2,4-hexadienal (≥95%). Dichloromethane (anhydrous, ≥99.8%) and pentane (anhydrous, ≥99%) used as diluents were also from Sigma-Aldrich.
Samples of the reference compounds were prepared by dilution of the individual compounds in Dichloromethane, except for a mixture of alkanes which were diluted in pentane. The concentration of stock solutions of each compound was 0.1 mg mL⁻¹ but the final dilution of each standard was individually modified to obtain good reproducibility of retention times.