Goat anti mouse igg fitc

Immunophenotypic analysis of BM-MSCs, AT-MSCs and UC-MSCs was performed at P3 with the FACS Calibur flow cytometer (BD, Franklin Lakes, NJ, USA) using the following antibodies: mouse anti-rat CD90-FITC (clone OX7, Caltag Laboratories, Burlingame, California, USA), mouse anti-human CD34-FITC (clone 581, BD, USA), mouse anti-human CD105-FITC (clone SN6, Abcam, San Francisco, California, USA), mouse anti-horse CD44 (clone CVS18, AbD Serotec, Kidlington, Oxfordshire, UK) and mouse anti-horse MHC class II monomorphic (clone CVS20, AbD Serotec, UK). For the unconjugated primary markers, the secondary antibody goat anti-mouse IgG-FITC (AbD Serotec, UK) was used. The protocols used were those described by the manufacturers.

All chemicals were from Sigma-Aldrich Handels GmbH (Vienna, Austria) unless noted otherwise. Cyclic olefin polymer (COP; Zeonor, Tokyo, Japan) foils with a thickness of 188 µm were obtained from microfluidic ChipShop GmbH (Jena, Germany). Positive photoresist G2 S1818 and developer ma-D 331S were obtained from micro resist technology GmbH (Berlin, Germany). Bovine serum albumin (BSA) was obtained from SERVA Electrophoresis GmbH (Heidelberg, Germany). Streptavidin-Cy5 and biotinylated epidermal growth factor (EGF) were purchased from Life Technologies (Vienna, Austria). EGFR-GFP was a kind gift from Alexander Sorking (Addgene 32751) [20 (link)]. FLAG peptide (DYDDDK) with N-terminal biotin and 4,7,10-trioxa-1,13-tridecanediamino succinic acid (Ttds) linker was synthesized by JPT Peptide Technologies (Berlin, Germany). Anti-FLAG antibody was obtained from Agilent (Vienna, Austria) and labeled using an Alexa Fluor 488 antibody labeling kit (Life Technologies). Goat anti-mouse IgG:FITC was obtained from AbD Serotec (Bath, UK), and biotin mouse anti-human CD71 was obtained from BD Biosciences (San Jose, CA, USA). RPMI 1640 medium, fetal bovine serum (FBS), L-glutamine, Genectin^TM Selective Antibiotic (G418), penicillin, and streptomycin were obtained from ThermoFisher Scientific (Waltham, MA, USA). Milli-Q water (18.2 MΩ) was used throughout.