Mouse cell removal beads

Manufactured by Miltenyi Biotec

Mouse cell removal beads are magnetic beads designed for the depletion of mouse cells from cell suspensions. They can be used to isolate non-mouse cells from mixed cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Midimacs separator, by Miltenyi Biotec (3 mentions) Facsaria fusion 2, by BD (1 mentions)

3 protocols using mouse cell removal beads

Isolating human cells from mixed samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dissociated cell pellets were resuspended in 160 μL FACS
buffer (0.5% BSA in 1X DPBS) + 40uL Mouse cell removal beads (Miltenyi) and
incubated at 4°C for 15 min. The resulting samples were then isolated
using LS columns and the MidiMACs separator (Miltenyi), the human cells were
collected in the flow through. Using centrifugation (10 min, 400xg), the
cells were pelleted and resuspended to 1,000 cells per microliter in FACS
buffer for scRNA-seq library preparation, according to the previous study
(Hasselmann et al., 2019 (link)).

Jin M., Xu R., Wang L., Alam M.M., Ma Z., Zhu S., Martini A.C., Jadali A., Bernabucci M., Xie P., Kwan K.Y., Pang Z.P., Head E., Liu Y., Hart R.P, & Jiang P. (2022). Type I Interferon Signaling Drives Microglial Dysfunction and Senescence in Human iPSC Models of Down Syndrome and Alzheimer’s Disease. Cell stem cell, 29(7), 1135-1153.e8.

+ Open protocol

+ Expand

Enrichment of Human Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dissociated cell pellets were resuspended in 160uL FACS buffer (0.5% BSA in 1X DPBS) + 40uL Mouse cell removal beads (Miltenyi) and incubated at 4°C for 15 minutes. Samples were then separated using LS columns and the MidiMACs separator (Miltenyi) and the human cells were collected in the flow through. Cells were pelleted via centrifugation (10 minutes, 400xg) and resuspended to ~1,000 cells per microliter in FACS buffer, according to counts performed on a hemocytometer.

Hasselmann J., Coburn M.A., England W., Velez D.X., Shabestari S.K., Tu C.H., McQuade A., Kolahdouzan M., Echeverria K., Claes C., Nakayama T., Azevedo R., Coufal N.G., Han C.Z., Cummings B.J., Davtyan H., Glass C.K., Healy L.M., Gandhi S.P., Spitale R.C, & Blurton-Jones M. (2019). Development of a chimeric model to study and manipulate human microglia in vivo. Neuron, 103(6), 1016-1033.e10.

+ Open protocol

+ Expand

Isolation of Human Microglia Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dissociated cell pellets were resuspended in 160 μL FACS buffer (0.5% BSA in 1X DPBS) + 40 μL Mouse cell removal beads (Miltenyi) and incubated at 4 °C for 15 min. Magnetically labeled cells were then separated using LS columns and the MidiMACs separator (Miltenyi) while the unlabeled human cells were collected in the flow through. Isolated human cells were pelleted via centrifugation and prepared for cell sorting by resuspending in 400 μL of FACS buffer containing Hoescht 33342 (1:400) as a viability marker. For separation of human microglia expressing either endogenous GFP or RFP, samples were sorted on a FACS ARIA Fusion II (BD Biosciences) using the 70 um nozzle at the lowest flow rate. After removing doublets, by both forward and side scatter parameters, and selecting for live cells (Hoescht 33342^-), both GFP (GFP⁺RFP^-) and RFP (GFP^-RFP⁺) cells were gated on and sorted into individual tubes. For each cell type, 15,000 cells were sorted into collection tubes containing 5uL of FACS buffer, resulting in a final volume of ~20 μL.

McQuade A., Kang Y.J., Hasselmann J., Jairaman A., Sotelo A., Coburn M., Shabestari S.K., Chadarevian J.P., Fote G., Tu C.H., Danhash E., Silva J., Martinez E., Cotman C., Prieto G.A., Thompson L.M., Steffan J.S., Smith I., Davtyan H., Cahalan M., Cho H, & Blurton-Jones M. (2020). Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer’s disease. Nature Communications, 11, 5370.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!