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Syringic acid

Manufactured by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, China, Spain, Japan, France, Sao Tome and Principe, Poland, India, Australia

Syringic acid is a phenolic compound that can be used as a chemical reagent in laboratory research and analysis. It serves as a standard reference material for analytical techniques such as chromatography and spectroscopy. The specific core function of syringic acid is to act as a calibration and measurement standard for the quantification of similar phenolic compounds in various samples.

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328 protocols using syringic acid

1

Phenolic Compound Standards for HPLC Analysis

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Phenolic compound standards: gallic acid, syringic acid, protocatechuic acid, caffeic acid, chlorogenic acid, naringenin, epicatechin, catechin, hesperetin, resveratrol, quercetin, rutin, ellagic acid, myricetin, kaempferol, luteolin, isorhamnetin, epigallocatechin, gallocatechin, gallocatechin gallate, epicatechin gallate, procyanidin A1, procyanidin A2, procyanidin B1, procyanidin B2, procyanidin C1, and corilagin were HPLC grade and purchased from Merck (St. Louis, MO, USA). Acetonitrile, formic acid, methanol, hexane, and water were HPLC/spectrum grade and purchased from Tedia (Fairfield, OH, USA). All other reagents and solvents used were analytical grade from Merck (St. Louis, MO, USA).
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2

Quantification of Phenolic Compounds

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Standards used for identification and quantification of phenolic compounds were gallic acid, syringic acid, catechin, chlorogenic acid, caffeic acid, p-coumaric acid, quercetin, rutin (quercetin-3-O-rutinoside), hyperoside (quercetin-3-O-galactoside), kaempherol, juglone (Merck, Darmstadt, Germany), ferulic acid and ellagic acid (Fluka-Honeywell, Charlotte, NC, USA). HPLC/MS grade acetonitrile and formic acid, analytical grade methanol and acetic acid were purchased from Avantor (Radnor, PA, USA). The bidistilled water was filtered through a 0.45 µm nylon membrane.
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3

Phenolic Standards Characterization Protocol

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Phenolic standards, namely, catechin, catechol, syringic acid, chlorogenic acid, cinnamic acid, ellagic acid, kaempferol, ferulic acid, gallic acid, rutin, caffeic acid, naringenin, benzoic acid, o-coumaric acid, p-hydroxybenzoic acid, pyrogallol, p-coumaric acid, quercetin, quinol, rosmarinic acid, and vanillic acid were bought from Merck KGaA (Darmstadt, Germany). The purities of standards were up to 99%. The solvents used were of analytical grade, dimethylsulfoxide (DMSO; Alfa Aesar GmbH & Co KG, Massachusetts, United States), orthophosphoric acid (H3PO4), ethanol, methanol, and acetonitrile HPLC-grade (Fisher Scientific International, Inc., Hampton, New Hampshire, United States).
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4

Optimized Extraction of Olive Phenolics

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The phenolic compounds were extracted from the olive pulp according to previously developed protocols [89 (link),90 (link)] with minor modifications. One gram of pulp from several drupes was extracted in 6 mL of DMSO and kept at 4 °C overnight. The extracts were filtered through a 0.45 μm nylon mesh. Syringic acid (Merck, Darmstadt, Germany) was added to each sample to be analyzed as an internal standard at a final concentration of 100 mg/L. The HPLC analyses of the phenolic extracts were conducted by a reversed-phase column using a HPLC-DAD, Agilent 1100 Series, Diode Array Detector [91 (link)], using a 2.6 µm Kinetex® Phenyl-Hexyl 100 Å, 100 × 4.6 mm LC Column (Phenomenex®, Torrance, CA, USA). Three external standard mixes by single net standards (Merck, Darmstadt, Germany) were prepared to identify the main phenols contained in olives. The quantification of each identified component has been carried out using the internal standard method.
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5

Phenolic Compounds: Analytical Standards

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Cyanidin, peonidin 3-o-glucoside, malvidin-3-o-glucoside, quercetin, quercetin 3-o-glucoside, coumaric acid, gallic acid, syringic acid, epicatechin, catechin, and kaempferol (all analytical standard) were from Merck (Milan, Italy). Methanol, ethanol, and acetonitrile (all LC/MS grade solvents), ortophospforic acid 85%, Folin–Ciocalteu reagent and sodium carbonate were bought from Carlo Erba (Milan, Italy). Water was purified through a Milli-Q system from Millipore (conductivity: 18.2 MΩ cm at 25 °C; Milford, MA, USA).
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6

Analytical Reagents for Biomedical Research

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Methanol (M), ethyl Acetate (EA), myricetin, gallic acid, quercetin, syringic acid, rutin, catechin, gentisic acid, cinnamic acid, kaempferol, luteolin, coumarin and apigenin were purchased from Merck (Darmstadt, Germany). Cefixime, ciprofloxacin dimethyl sulfoxide (DMSO), nutrient broth, nutrient agar, Coomassie brilliant blue and fetal bovine serum were obtained from Sigma-Aldrich, USA.
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7

Standardized Extraction and Derivatization of Phenolic Compounds

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All reagents and solvents used were minimally of analytical purity. Standard compounds, trans-caffeic acid (99%), vanillic acid (97%), syringic acid (97%), trans-pcoumaric acid (98%), trans-o-coumaric acid (98%) and trans-ferulic acid (98%) and solvents, tetrahydrofuran-THF (99.5%) and pyridine (99.9%), were supplied by Merck (Germany). Derivatization reagent N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA), HPLC-grade methanol (MeOH) and sodium hydroxide-NaOH (99%) were purchased from Sigma (USA). GC-grade toluene (99.5%) and hydrochloric acid-HCl (36.5%) were purchased from Carlo Erba (Italy). Dichloromethane-DCM was purchased from JT Baker (Germany), L-ascorbic acid (99.7%) was purcha-sed from Alkaloid (Macedonia) and EDTA was purchased from Kemika (Croatia). The water used was obtained from a Milli-Q water purification system.
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8

Antioxidant Capacity Assays Reagents

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Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonate) (ABTS; ≥98%), 2,2-diphenyl-1-picrylhydrazyl (DPPH radical), Folin-Ciocalteu's phenol reagent, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; 97%), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ; ≥98%), chlorogenic acid (CHA; 95%), salicylic acid (≥99%), p-coumaric acid (≥98%), sinapic acid (≥98%), syringic acid (≥95%), caffeic acid (≥98%), vanillin (99%), vanillic acid (≥97%), gallic acid (97.5-102.5%), starch from potato, 3,5-dinitrosalicylic acid (DNSA; ≥98%), sodium chloride (≥99.5%), and α-amylase from porcine pancreas (Type VI-B, ≥5 units/mg solid) were acquired from Sigma-Aldrich (Milan, Italy). Acetonitrile, formic acid and ultrapure water from Carlo Erba Reagents (Milan, Italy) were UPLC-MS grade.
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9

Antioxidant Capacity Determination Protocol

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Ethanol, Folin–Ciocalteu’s phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), aluminum chloride hexahydrate, sodium nitrite, (+)-catechin, gallic acid monohydrate, sodium acetate anhydrous, potassium chloride, hydrochloric acid, sodium carbonate anhydrous, oxlalic acid, tartaric acid, malic acid, lactate, acetic acid, citric acid, succinic acid, fumaric acid, fructose, glucose, sucrose, maltose, lactose, sorbitol, gallic acid, protocatechuic acid, catechol, catechin, chlorogenic acid, epigallocatechin gallate, caffeic acid, epicatechin, syringic acid, 4-methycatechol, epicatechin gallate, p-coumaric acid, ferulic acid, and rutin were purchased from Sigma (St. Louis, MO, USA).
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10

Comprehensive Metabolite Profiling Protocol

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Acetylcholine, acetyl-d3-L-carnitine hydrochloride, acetyl-Lcarnitine, adrenic acid, L-carnitine, Lcitrulline, dodecanoic acid, (-)-epicatechin, gallic acid, glycochenodeoxycholic acid, glycocholic acid, glycocholic acid-(glycyl-1-13C) monohydrate, α-hydroxyisobutyric acid, indole-3-acetic-2,2-d2 acid, Lisoleucine, 7-ketocholesterol, L-leucine, leukotriene B4, margaric acid, palmitic acid, Lphenylalanine, stearic acid, syringic acid, L-tryptophan, uric acid, and L-valine were purchased from Sigma-Aldrich (St. Louis, MO). 4-hydroxyhippuric acid was purchased from PhytoLab GmbH and Co KG (Vestenbergsgreuth, Germany), naringenin from Extrasynthèse (Genay, France), and arachidonic acid from Cymit Quimica (Barcelona, Spain). UHPLC-MS-grade methanol, acetone, formic acid, and HPLC-grade acetonitrile were purchased from Scharlau Chemie S.A. (Barcelona, Spain). Ultrapure water (Milli-Q) was obtained from a Milli-Q Gradient A10 system (Millipore, Bedford, MA).
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