Untreated 10 cm petri dishes

Bone marrow cells were collected from femurs and tibiae of 6‐ to 8‐week‐old C57BL/6 wild‐type (WT) or MSR1/SR‐A knock‐out mice (kindly provided by Siamon Gordon). The cells were treated with red blood cell lysis buffer (155 mM NH₄Cl, 12 mM NaHCO₃, 0.1 mM EDTA) and plated on tissue culture plastic (Corning Incorporated) for 3 days in DMEM (Gibco) containing 10% FBS, 2 mM glutamine, 100 units/ml penicillin–streptomycin (Gibco) and 20% L929 conditioned supplement. At day 3, the cells in supernatant were transferred to untreated 10‐cm Petri dishes (BD Biosciences) for 7 days for the differentiation into bone marrow‐derived macrophages (BMDMs). Then, BMDMs were treated by either IL‐4 (20 ng/ml, BD Pharmingen) for 48 h to get M2 (IL‐4) macrophages (Appendix Fig S1).

Bone marrow cells were collected from femurs and tibia of both male and female 6‐ to 8‐week old C57BL/6J or C57BL/6NTac wild‐type mice. Collected cells were treated with red blood cell lysis buffer (155 mM NH₄Cl, 12 mM NaHCO₃, 0.1 mM EDTA) and plated on untreated 10 cm cell culture dishes (BD Biosciences) in IMDM (Gibco) containing 10% heat‐inactivated FBS, 100 units/ml penicillin/streptomycin (Gibco) and 15% L929 conditioned supplement. After 24 h, the cells in supernatant were transferred to untreated 10 cm Petri dishes (BD Biosciences) for 7 days for the differentiation into bone marrow‐derived macrophages (BMDMs) (Heap et al, 2021 (link)).