MiR-NC mimics, miR-129-5p mimics, miR-NC antagonist, and miR-129-5p antagonist were bought from RiboBio (Guangzhou, P.R. China). For miR-129-5p overexpression or downregulation, miR-129-5p mimics or miR-129-5p antagonist was mixed with Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific), maintained for 15 minutes, and then added into culture medium of cells. Seventy-two hours after transfection, the cells were harvested and the RNA and/or protein was extracted for the following experiments.
Mir 129 5p antagonist
Manufactured by RiboBio
The MiR-129-5p antagonist is a laboratory reagent designed to inhibit the function of the microRNA miR-129-5p. It is a synthetic oligonucleotide that binds to and neutralizes miR-129-5p, preventing it from interacting with its target mRNAs. The MiR-129-5p antagonist is intended for use in research applications to study the biological roles and functions of miR-129-5p in various cellular and molecular processes.
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Lab products found in correlation
2 protocols using mir 129 5p antagonist
1
MiRNA Overexpression and Knockdown Protocol
2
Molecular Mechanisms of NEAT1 Regulation
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Transfection was conducted via Lipofectamine 2000 in accordance to the manufacturer’s specifications. A NEAT1 overexpression plasmid, pcDNA3.1-NEAT1, was commercially constructed by Genechem Co., Ltd. (Shanghai, China). The NEAT1 siRNA sequences used were 5′-GUGAGAAGUUGCUUAGAAACUUU-3′ and 5′-GGAAAGUUUCUAAGCAACUUCUCAC-3′. The siRNA for Bcl-2 was 5′-AACAUCGCCCUGUGGAUGACU-3′. The control counterparts were 5′-GUACCUGACUAGUCGCAGAAG-3′ and 5′-UCUGCGACUAGUCAGGUACGG-3′. All sequences were synthesized and bought from GenePharma. On the other hand, the miR-129-5p mimic, miR-129-5p antagonist, and controls were purchased from RiboBio [38 (link)].
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