Biotin rabbit anti mouse igg

Quercitrin (high pressure liquid chromatography, purity > 98%) was purchased from Shanghai Tongtian Biotechnology Co., Ltd (Jiangsu, China), anti-Iba1 antibody was obtained from Wako (Osaka, Japan), biotin-rabbit antimouse IgG was purchased from Life Technologies (New York, USA), streptavidin conjugating horseradish peroxidase (SHP) was purchased from Invitrogen (New York, USA), protein precipitation solution and nuclei lysis solution were purchased from Promega (Wisconsin, USA), thioflavin-s and 3,3′-diaminobenzidine (DAB) were purchased from Sigma (Shanghai, China), agarose was purchased from Biowest (Loire Valley, France) and bio-plexpro mouse chemokine 23-plex panel was purchased from Bio-Rad (California, USA).

MCL was isolated from Michelia compressa (Magnoliaceae), and HPLC purity was ≥99%. Its molecular weight is 248.32 KDa, and the molecular formula is C₁₅H₂₀O₄ (Fig. S1). Primary Iba1 antibody was ordered from Wako (Japan). Anti-GFAP antibody was ordered from Abcam (Japan). Biotin-rabbit anti-mouse IgG and SYBR master mix were ordered from Life Technologies (USA). The second antibody and TRIzol were ordered from Invitrogen (USA). 3,3′-diaminobenzidine (DAB), thioflavin-s, LPS and BCA protein assay reagents were ordered from Sigma (Germany). Genomic DNA isolation solutions were ordered from Promega (USA). Agarose was ordered from Biowest (France). Bio-Plex Pro mouse chemokine panel 23-plex was ordered from Bio-Rad (USA). Reverse transcriptase was ordered from Takara (Japan). RIPA was ordered from Beyotime (China). A phosphatase inhibitor was ordered from Roche (Switzerland). AF488-labeled goat anti-rabbit antibody was ordered from Molecular Probes (USA).

AS-IV (Cat.83207-58-3, and HPLC purity >98%): Tauto Biotech (Shanghai, China). LPS (E.coli 0111:B4) (Cat.437620), 3,3′-diaminobenzidine (DAB) (Cat.D8001), thioflavin-S (Cat.T189) and BCA protein assay reagents (Cat.BCA1): Sigma (China). Anti-Iba1 primary antibody (Cat.012-26723): Wako (Japan). Biotin-rabbit anti-mouse IgG (Cat.7074): Life Technologies (USA). Streptavidin conjugated-horseradish peroxidase (Cat.RABHRP3) and TRIzol: Invitrogen (USA). p-P65 (Ser536) (Cat.3033), p-IκBα (Ser32/36) (Cat.9246), AF488 fluorescent antibody (Cat.15008) and corresponding antibodies against total proteins: Cell Signaling Technology (Shanghai, China). Protein and nucleic acid precipitation solution (Cat.A7124) (Cat.A7943): Promega Biotech (China). Agarose (Cat.A9539): Biowest (France). Nitric oxide (NO) assay kit (Cat.S0025), RIPA and CCK8 kit: Beyotime (China). Reverse transcriptase (Cat.2690A): Takara (China). SYBR selection master mix: Life Technologies (USA). DMEM culture medium, fetal bovine serum: Gibco (USA).