Ft ir spectrometer

Melting points were taken in an open capillary tube on a Stuart melting point apparatus (Stuart Scientific, Redhill, UK) and were uncorrected. The IR spectra of the compounds were recorded on FTIR Shimadzu spectrometer (Shimadzu, Tokyo, Japan). ¹H NMR and 13C NMR spectra were recorded on a Varian Mercury Plus Oxford (300 MHz for ¹H-NMR and 75 MHz for 13C-NMR) spectrometer (Varian Inc., Palo Alto, CA) using TMS as an internal Standard and DMSO-d₆ as solvent. Mass spectra were run on HP Model MS-5988 (Hewlett Packard, Palo, Alto, CA). Microanalyses were obtained on a Carlo Erba 1108 Elemental Analyzer (Heraeus, Hanau, Germany). All values were within ±0.4% of the theoretical values. Purity of the compounds was checked by TLC on pre-coated SiO₂ gel (HF254, 200 mesh) aluminium plates (Merk, Darmstadt, Germany). A developing solvent system of chloroform/methanol (8:2) was used and the spots were visualised under UV light. IR, ¹H NMR, 13C NMR, Mass and elemental analysis were consistent with the assigned structures. Starting sulfanilamide and all reagents used were of analytical grade and were purchased from Sigma (St. Louis, MO).

Melting points were recorded uncorrected in open capillary tubes using Stuart melting point apparatus (Stuart Scientific, Redhill, UK). Infrared (IR) spectra of all newly synthesized compounds were reported using FTIR Shimadzu spectrometer (Shimadzu, Tokyo, Japan). Bruker 400 NMR spectrometer (Bruker Bioscience, MA, USA) was used at 400 and 100 MHz for recording ¹H-NMR and ¹³C-NMR spectra, respectively. TMS was used as an internal standard, while deuterated DMSO was used as a solvent. HP Model MS-5988 (Hewlett Packard, Palo, Alto, California, USA) was used to determine mass spectra. To obtain microanalyses values, a Carlo Erba 1108 Elemental Analyzer (Heraeus, Hanau, Germany), was used. For checking of reactions’ Completion, Pre-coated SiO₂ gel (200 mesh, HF254) aluminum plates (Merk, Darmstadt, Germany) were used as TLC. Where a developing solvent system of chloroform/methanol (6.5:3.5) was used. Spots were visualized under UV light. Spectral and elemental analysis were in consistent with the postulated structures. For purity inspection, HPLC was used (Agilent 1260 Infinity apparatus) on ZORBAX Eclipse Plus C18 column (4.6 × 100 mm, 3.5 µm), with a mobile phase acetonitrile:water mixture (60:40) at a flow rate of 1.0 mL/min and the detection wavelength was set at 250 nm.

Uncorrected melting points were recorded using a Stuart melting point device (Stuart Scientific, Redhill, UK) and were transported in open capillary tubes. The infrared (IR) spectra of the substances were captured using an FTIR Shimadzu spectrometer (Shimadzu, Tokyo, Japan). TMS was utilised as an internal Standard and DMSO-d6 as the solvent for recording 1H NMR and 13C NMR spectra with a Bruker (400 MHz for 1H NMR and 100 MHz for 13C NMR) spectrometer. On the HP Model MS-5988, mass spectra were conducted (Hewlett Packard, Palo, Alto, Calofornia, USA). For acquiring the results of the microanalyses, a Carlo Erba 1108 Elemental Analyzer (Heraeus, Hanau, Garmany) was employed. In order to check the completion of the reaction, pre-coated SiO2 gel (HF254, 200 mesh) aluminium plates (Merk, Daemstadt, Germany) were employed as the TLC. CAS number for reagents used are mentioned between brackets following each reagent.