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5 protocols using sodium lauryl sulfate

1

Quantitative Proteomic Analysis of Cells

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GBN standard products were acquired from Shanghai Yuanye Biotechnology Co., Ltd. (batch number G006171216). Ammonia, iodoacetamide, and triethylammonium bicarbonate buffer were all procured from Sigma. Acetone and sodium lauryl sulfate were purchased from Shanghai Sinopharm. TMT 16Plex, Protein Ladder, Protease Inhibitor Cocktail, Pierce™ BCA Protein Assay Kit, NUPAGE 10% BT GEL 1.0MM 12 W, and Bond-Breaker™ TCEP Solution (TCEP) were produced by Thermo Fisher Scientific. Acetonitrile, methanol, formic acid, and other reagents required for the experiments were all of analytical grade. American Sigma Company provided dimethyl sulfoxide reagents. Fetal bovine serum (FBS) was sourced from Gibco, United States (10099-141). Beijing Soleibao Technology Co., Ltd., provided 0.25% trypsin–EDTA digestion solution and EDTA-free trypsin digestion solution.
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2

Development and Evaluation of Glipizide Tablets

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Glipizide tablet (Glucotrol, 5 mg), manufactured by Pfizer U.S. Pharmaceuticals Group (New York, USA), was the reference listed drug (RLD). Stearic acid (≥99.5%, SA) and silicon dioxide (≥99.0%) were purchased from Huzhou Prospect Pharmaceutical Co., Ltd. (Zhejiang, China). Lactose (≥99.5%), microcrystalline cellulose (≥99.0%, MCC) and starch (≥99.5%) were produced by Shanghai Yunhong Pharmaceutical Excipients Co., Ltd. (Shanghai, China). Glipizide (≥99.5%) and sodium lauryl sulfate (≥99.0%, SLS) were supplied by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China.). Pure water was produced by water purification system (Academic, Millipore, USA).
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3

Characterization of Biosynthesized AgNPs

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AgNPs (⌀5 and 50 nm) were purchased from Xi’an Ruixi Biological Technology Co., Ltd. (Xi’an City, China). Triton™ X100, sodium lauryl sulfate and soy peptone were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Agar, pepsin, and Masson’s trichrome staining reagent were purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China). Tryptone was purchased from OXOID (Shanghai, China), calcein was purchased from Yeasen Biotech Co., Ltd. (Shanghai, China), and Cell Counting Kit-8 (CCK8) was obtained from APExBIO Technology, LLC (USA). Trypsin was purchased from Sigma–Aldrich (USA). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent was purchased from GlpBio (USA).
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4

Pomelo Fruitlets Chemical Analysis

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Pomelo fruitlets were obtained from Zhangzhou Pomelo Township Food Co., Ltd. (Zhangzhou, China). Monosodium phosphate, sodium lauryl sulfate, ethylenediaminetetraacetic acid, concentrated hydrochloric acid (HCl), concentrated sulfuric acid (H2SO4), sodium hydroxide (NaOH), hydrogen peroxide (H2O2), and anhydrous magnesium sulfate were of analytical grade, and bought from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
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5

Methotrexate-loaded Solutol-HS 15 Nanoparticles

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CM and Freund’s complete adjuvant (FCA) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). MTX was purchased from Jiangsu Hengrui Medicine Co., Ltd. (Lianyungang, People’s Republic of China). Solutol-HS 15 was supplied by BASF Co., Ltd. (Shanghai, People’s Republic of China). Pepsin, pancreatin, and sodium lauryl sulfate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). Hydroxypropyl methyl cellulose E5 was obtained from Colorcon Co., Ltd. (Shanghai, People’s Republic of China). A MaxVision™ HRP-Polymer anti-Rabbit IHC Kit was from MXB Co., Ltd. (Fuzhou, People’s Republic of China). Soybean oil was from Jinlongyu Co., Ltd. (Taiyuan, People’s Republic of China). TNF-α and IL-1β enzyme-linked immunosorbent assay kits were supplied by Boster Co., Ltd. (Wuhan, People’s Republic of China). Other reagents were of analytical grade.
Male Sprague Dawley rats with body weights of 150±20 g were from the Laboratory Animal Center, General Hospital of Nanjing Military Region (SYXK (su) 2003–0032). All of the animal procedures were reviewed and approved by the Ethics Committee and the Institutional Animal Care and Use Committee of the Anhui University of Traditional Chinese Medicine (Hefei, People’s Republic of China).
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