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Apoptag s7100

Manufactured by Merck Group
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The ApopTag S7100 is a laboratory equipment product that is designed to detect and analyze apoptosis, a programmed cell death process. It provides a reliable and efficient method for researchers to study and quantify apoptosis in various cell types and experimental models.

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Lab products found in correlation

Ab7842, by Merck Group (2 mentions) Ab30788, by Abcam (2 mentions) Ab87483, by Abcam (2 mentions) D9564, by Merck Group (2 mentions) M0744, by Agilent Technologies (2 mentions) M7240, by Merck Group (2 mentions) G2654, by Abcam (2 mentions) Phospho histone h3, by Merck Group (2 mentions) Optical microscope, by Olympus (1 mentions)

3 protocols using apoptag s7100

1

Detailed Pancreatic Tissue Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the end of the follow-up period, mice were sacrificed, pancreata were
harvested, fixed, and embedded in paraffin 6 h post-injection with BrdU (100
mg/kg/bw). Sections were stained using antibodies to BrdU (Dako, M0744, 1:100),
Ki67 (Dako, M7240, 1:100), phosphohistone H3 (Millipore, 06–570, 1:250),
or insulin (Abcam, ab7842, 1:500), glucagon (Sigma, G2654, 1:8000), somatostatin
(Abcam, ab30788, 1:1000), β2M (Abcam, ab87483, 1:80), serum anti-ZnT8
(c-term), 1:500, serum anti-phogrin (C-term), 1:250 (gift from H. Davidson, Univ
Colorado) and appropriate secondary antibodies and counterstained with DAPI
(Sigma, D9564, 1:6600). Cell death was detected by TUNEL assay (ApopTag S7100;
Chemicon). At least 1,000–2,000 β-cell nuclei were counted per
animal, and data were expressed as a percentage of BrdU+, Ki67+, pHH3+ or TUNEL+
β-cells. Insulitis was evaluated as reported previously38 (link). β-cell mass was
evaluated by point-counting morphometry on immunofluorescence-stained sections
of the pancreas5 (link).
Dirice E., Kahraman S., De Jesus D.F., El Ouaamari A., Basile G., Baker R.L., Yigit B., Piehowski P.D., Kim M.J., Dwyer A.J., Ng R.W., Schuster C., Vethe H., Martinov T., Ishikawa Y., Teo A.K., Smith R.D., Hu J., Haskins K., Serwold T., Qian W.J., Fife B.T., Kissler S, & Kulkarni R.N. (2019). Increased β-cell proliferation before immune cell invasion prevents progression of type 1 diabetes. Nature metabolism, 1(5), 509-518.
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2

Detailed Pancreatic Tissue Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the end of the follow-up period, mice were sacrificed, pancreata were
harvested, fixed, and embedded in paraffin 6 h post-injection with BrdU (100
mg/kg/bw). Sections were stained using antibodies to BrdU (Dako, M0744, 1:100),
Ki67 (Dako, M7240, 1:100), phosphohistone H3 (Millipore, 06–570, 1:250),
or insulin (Abcam, ab7842, 1:500), glucagon (Sigma, G2654, 1:8000), somatostatin
(Abcam, ab30788, 1:1000), β2M (Abcam, ab87483, 1:80), serum anti-ZnT8
(c-term), 1:500, serum anti-phogrin (C-term), 1:250 (gift from H. Davidson, Univ
Colorado) and appropriate secondary antibodies and counterstained with DAPI
(Sigma, D9564, 1:6600). Cell death was detected by TUNEL assay (ApopTag S7100;
Chemicon). At least 1,000–2,000 β-cell nuclei were counted per
animal, and data were expressed as a percentage of BrdU+, Ki67+, pHH3+ or TUNEL+
β-cells. Insulitis was evaluated as reported previously38 (link). β-cell mass was
evaluated by point-counting morphometry on immunofluorescence-stained sections
of the pancreas5 (link).
Dirice E., Kahraman S., De Jesus D.F., El Ouaamari A., Basile G., Baker R.L., Yigit B., Piehowski P.D., Kim M.J., Dwyer A.J., Ng R.W., Schuster C., Vethe H., Martinov T., Ishikawa Y., Teo A.K., Smith R.D., Hu J., Haskins K., Serwold T., Qian W.J., Fife B.T., Kissler S, & Kulkarni R.N. (2019). Increased β-cell proliferation before immune cell invasion prevents progression of type 1 diabetes. Nature metabolism, 1(5), 509-518.
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3

Apoptosis Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
A TUNEL assay was performed for all groups according to the manufacturer’s protocol (ApopTag S7100; Chemicon International, Billerica, MA). Counting apoptotic cells in 10 arbitrarily selected fields at ×400 magnification under optical microscope (Olympus, Japan). The apoptotic rate (per ×400 microscopic fields) was calculated as number of apoptotic cells ×100/total number of cells.
Qu S., Guo Y., Huang S.T, & Zhu X.D. (2017). Inhibition of STAT1 sensitizes radioresistant nasopharyngeal carcinoma cell line CNE-2R to radiotherapy. Oncotarget, 9(9), 8303-8310.
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