Uas mcherry

The w¹¹¹⁸ (used as wild-type strain in this study), dKlf15^NN (CG2932, FBgn0025679; previously known as Bteb2^f06447 was described in the study by Ivy et al^{17 (link)}), Dorothy-Gal4 (dot-Gal4, originally described in the study by Kimbrell et al^{19 (link)}), UAS-mCherry (TRiP control line), dSparc^MI00329 (with an MiMIC insertion in the 5-prime region of the dSparc locus; as described in the study by Venken et al^{20 (link)}), and Tub-Gal80^ts lines were all from the Bloomington Stock Center (Bloomington, IL). The HandC-Gal4 (Hand-Gal4) line was described in the study by Sellin et al^{21 (link)}). The 2 RNAi lines for knocking-down dKlf15 were from the Vienna Drosophila RNAi stock Center (with a targeting hairpin inserted into the second chromosome, VDRC) and Bloomington (Klf15^JF02420, a Harvard TRiP line with a dKlf15 targeting hairpin inserted into the third chromosome). All genetic combinations were generated by standard crosses. Generation of TARGET flies (Hand-Gal4; Tub-Gal80^ts) was achieved by standard crosses.^{22 (link)}

Fly lines w¹¹¹⁸, FRT82, Labial^RNAi (BL26753), UAS-mcherry (BL38245), UAS-Tkv^QD (BL36536), UAS-Dpp (BL1486), UAS-Ubx (BL911), Ubx¹ (BL529), Ubx^RNAi line 1 (BL31913), Ubx^RNAi line 2 (BL34993), Dad^RNAi (BL33759) were obtained from Bloomington Drosophila Stock Center. esg-Gal4, UAS-GFP was a gift from Shigeo Hayashi; FRT82B, Dad²¹² from Hannele Ruohola-Baker; Btl-Gal4^ts, UAS-GFP from Dirk Bohmann; Dad::nlsGFP from Georgios Pyrowolakis; Su(H)-GBE-lacZ from Sarah Bray; esg^tsF/O (esgGal4, tubG80ts, UAS-GFP; UAS-flp, act > STOP > Gal4) from Huaqi Jiang; UAS::Dad from Thomas Kornberg; NP1::Gal4 from Dominique Ferrandon; MARCM82 (hsFlp; tub-Gal4, UAS-GFP; FRT82, tubGal80) from Norbert Perrimon.
Flies were cultured on yeast/molasses-based standard fly food (Recipe: 10 L H2O, 138 g agar, 220 g molasses, 750 g malt extract, 180 dry yeast, 800 g corn flour, 100 g soy flour, 62.5 ml propionic acid, 20 g Methyl 4-Hydroxybenzoate, and 72 ml ethanol) at 25 °C with a 12 h light/dark cycle. For TARGET (tubGal80ts) experiments, flies were raised at 18 °C to allow Gal80 to inhibit Gal4, and 3–4 days after eclosion shifted to 29 °C to inhibit Gal80 and to allow Gal4 to drive UAS-linked transgene expression.