Following stimulation of LAD2 cells with U2987MG-conditioned medium, the histamine levels (Oxford Biomedical Research, MI, USA) and TNF-α levels (R&D Systems, Abingdon, UK) in the media was measured by sandwich ELISA following the manufacturer's protocol. Both ELISA were done in triplicates for each stimulation assay.
Histamine
Manufactured by Oxford Biomedical Research
Sourced in United States
Histamine is a chemical compound that plays a crucial role in various physiological processes. It functions as a neurotransmitter and is involved in immune system responses. The core function of Histamine is to facilitate signal transmission and regulate biological activities within the body.
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Lab products found in correlation
8 protocols using histamine
1
Histamine and TNF-α Profiling of LAD2 Cells
2
Quantification of Serum Immunological Markers
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Before sacrifice, blood samples were collected after puncturing the heart. Cells were removed from plasma by centrifugation for 10 min at 6000× g at 4 °C. The serum levels of immunoglobulin E (IgE) (Bethyl Laboratories Inc., Montgomery, TX, USA) and histamine (Oxford Biomedical research, Inc., Oxford, MI, USA) were measured using specific ELISA kits according to the manufacturer’s instructions. After sacrifice, dorsal skin biopsies were taken from each group. The skin tissues were homogenized and analyzed for the levels of interleukin-17A (IL-17A) using specific ELISA kits (BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions.
3
Quantifying Immune Biomarkers in Samples
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The serum levels of IgE (BioLegend, San Diego, CA, USA), histamine (Oxford Biomedical Research, Rochester Hills, MI), and TSLP (R&D Systems Inc., Minneapolis, MN, USA) were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits, in accordance with the manufacturer's instructions.
4
Quantification of Allergy Biomarkers
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Serum levels of IgE (BioLegend, San Diego, CA, USA), histamine (Oxford Biomedical Research, Rochester Hills, MI, USA), and TSLP (R&D Systems Inc., Minneapolis, MN, USA) were measured using commercial ELISA kits, according to the manufacturers’ instructions.
5
Plasma IgE and Histamine ELISA
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The plasma levels of IgE (Bethyl Laboratories Inc., U.S.A.) and histamine (Oxford Biomedical Research, U.S.A.) were measured by ELISA accordance with the manufacturer’s instructions.
6
Plasma Histamine and IgE Analysis
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Blood samples were drawn from the mice, separated by centrifugation at 10000 × g for 10 min at 4°C, and stored at -80°C. The levels of histamine (Oxford Biomedical Research, Oxford, MI, USA) and IgE (Bethyl Laboratories Inc., Montgomery, TX, USA) were measured in the plasma using ELISA kits, according to the manufacturer’s instructions.
7
Quantifying Plasma IgE and Histamine
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The plasma levels of IgE (Bethyl Laboratories Inc., USA) and histamine (Oxford Biomedical Research, USA) were quantified using ELISA kits according to the manufacturer's instructions.
8
Quantifying Histamine and IgE Levels
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The plasma levels of histamine (Oxford Biomedical Research, Rochester Hills, MI) and IgE (Bethyl Laboratories Inc., Montgomery, TX) were measured using EIA kits in accordance with the manufacturer's instructions.
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