H. influenzae Rd KW20 strains (including mutants) were inoculated at an OD600 of 0.1 in sBHI [with 50 μM beclomethasone dipropionate (Sigma-Aldrich) if used]. Cultures were harvested during mid-log phase (OD600 = 0.7), and RNA was extracted using the High Pure RNA Isolation Kit (Roche). For the in vivo RNA samples, infected animals as described above were euthanized < 72 h post-infection. RNA was isolated from airway homogenate pellets using TRIzol according to the manufacturer’s instructions. Samples were treated with DNase, and then rRNA was removed from 1 to 5 μg of total RNA using the Ribo-Zero kit for Gram-negative bacteria (Epicentre). rRNA-depleted samples were concentrated by EtOH precipitation.
Beclomethasone dipropionate
Manufactured by Merck Group
Sourced in Italy
Beclomethasone dipropionate is a synthetic glucocorticoid used as a topical anti-inflammatory agent. It is commonly used in various pharmaceutical and laboratory applications, including the formulation of inhalation solutions and topical preparations. The product is designed to provide effective treatment for inflammatory conditions, although its specific intended use should be determined based on professional medical advice.
Automatically generated - may contain errors
Lab products found in correlation
Ribozero kit for gram negative bacteria, by Illumina (1 mentions)
High pure rna isolation kit, by Roche (1 mentions)
Curcumin, by Merck Group (1 mentions)
Prochloraz, by Merck Group (1 mentions)
Fluticasone propionate, by Merck Group (1 mentions)
Forskolin, by Merck Group (1 mentions)
Mucin, by Merck Group (1 mentions)
Reagent grade salts, by Merck Group (1 mentions)
Sephadex g 50 medium, by Merck Group (1 mentions)
Milli q gradient a10 system, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Pluronic f127, by BASF (1 mentions)
5 protocols using beclomethasone dipropionate
1
Transcriptome Analysis of H. influenzae
2
Topical Curcumin Delivery Formulation
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Beclomethasone dipropionate, curcumin, Kolliphor P188 (Poloxamer 188, P188) were obtained from Sigma Aldrich (Milan, Italy). All the other products were of analytical grade.
3
Pharmacological Modulation of Steroid Receptors
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Forskolin, prochloraz, fluticasone propionate, and beclomethasone dipropionate were obtained from Sigma-Aldrich (Gillingham, UK). Synacthen® (tetracosactide acetate) was obtained from Mallinckrodt Pharmaceuticals.
4
Formulation and Characterization of Lipid-Based Nanoparticles
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Lipoid S75 (S75), a mixture of soybean phospholipids (~70% phosphatidylcholine, 9% phosphatidylethanolamine and 3% lysophosphatidylcholine), triglycerides and fatty acids, was kindly provided by AVG S.r.l. (Garbagnate Milanese, Milan, Italy), local supplier for Lipoid GmbH (Ludwigshafen, Germany). Sodium hyaluronate was purchased from DSM Nutritional Products AG Branch Pentapharm (Rheinfelden, Switzerland). Beclomethasone dipropionate, mucin and all the other reagents were of analytical grade and were purchased from Sigma-Aldrich (Milan, Italy). Reagents and plastics for cell culture were purchased from Life Technologies Europe (Monza, Italy).
5
Lipid-based Nanoformulation Preparation
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All chemicals were purchased of the highest purity available and were used without further purification. Ethanol, methanol, Sephadex G-50 medium, cholesterol, beclomethasone dipropionate (BDP), the reagent grade salts for the 50 mM K-phosphate, 100 mM KCl (pH 7.0) buffer solutions and sodium cholate were purchased from Sigma-Aldrich. 1,2-Dipalmitoyl-sn-glycero-3-
ammonium salt (LissRhod PE) were purchased from Avanti Polar Lipids. Lipoid S100 (LS100) was from Lipoid (Lipoid GmbH, Germany). According to the manufacturer's instructions, LS100 is constituted by approximately 100% soybean phosphatidylcholine (PC). Pluronic F127 ®
[poly(ethylene oxide) -poly(propylene oxide) -poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer, PF127] was a kind gift of BASF (Ludwigshafen, Germany). Sterile filters of cellulose acetate of 0.2 µm were from Advantec. All aqueous solutions were prepared by using water obtained from a Milli-Q gradient A-10 system (Millipore, 18.2 MΩ cm, organic carbon content ≤4 μg/L).
ammonium salt (LissRhod PE) were purchased from Avanti Polar Lipids. Lipoid S100 (LS100) was from Lipoid (Lipoid GmbH, Germany). According to the manufacturer's instructions, LS100 is constituted by approximately 100% soybean phosphatidylcholine (PC). Pluronic F127 ®
[poly(ethylene oxide) -poly(propylene oxide) -poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer, PF127] was a kind gift of BASF (Ludwigshafen, Germany). Sterile filters of cellulose acetate of 0.2 µm were from Advantec. All aqueous solutions were prepared by using water obtained from a Milli-Q gradient A-10 system (Millipore, 18.2 MΩ cm, organic carbon content ≤4 μg/L).
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