FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
Fitc conjugated anti mouse ifn γ clone xmg1
Manufactured by BD
The FITC-conjugated anti-mouse IFN-γ (clone XMG1.2) is a laboratory reagent used to detect and quantify mouse interferon-gamma (IFN-γ) in biological samples. It is a fluorescently labeled monoclonal antibody that specifically binds to mouse IFN-γ, allowing for its identification and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated cd25, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated gr 1, by Thermo Fisher Scientific (2 mentions)
Pe conjugated cd11b, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated anti mouse cd4, by Thermo Fisher Scientific (2 mentions)
Fitc and pe conjugated anti mouse cd8a clone 53, by BD (2 mentions)
Purified anti mouse cd16 32 fc block, by BD (2 mentions)
Cisplatin, by Merck Group (1 mentions)
Mannitol, by Merck Group (1 mentions)
Tween 40, by Merck Group (1 mentions)
3 protocols using fitc conjugated anti mouse ifn γ clone xmg1
1
Multicolor Flow Cytometry Analysis
2
Multicolor Flow Cytometry Analysis
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FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7, catalog no. 553031 and 553032 respectively), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2, catalog no. 554411) these antibodies were acquired from BD Pharmingen (San Diego, CA). Purified anti-mouse CD16/32 (Fc Block ™, catalog no. 553141) was purchased from BD Pharmingen. FITC-conjugated anti-mouse CD4 (catalog no. 11-0042-82), APC-conjugated CD25 (catalog no. 17-0251-82), FITC-conjugated Gr-1 (catalog no. 11-5931-82) and PE-conjugated CD11b (catalog no. 12-0112-82) antibodies were acquired from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 peptide, E7aa49-57 (RAHYNIVTF), was synthesized by Macromolecular Resources (Denver, CO) with ≥80 % purity. PE-conjugated HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility.
3
HPV16 Peptide Synthesis and Immune Assays
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The H-2Kb-restricted HPV16 E6aa50-57 peptide, YDFAFRDL, and H-2Db-restricted HPV16 E7aa49-57 peptide, RAHYNIVTF were synthesized by Macromolecular Resources (Denver, CO) at a purity of ≥80%. FITC or PE-conjugated anti-mouse CD4 (clone RM4-5) and CD8a (clone 53.6.7), and FITC-conjugated anti-mouse IFN-γ (clone XMG1.2) antibodies were purchased from BD Pharmingen (San Diego, CA). PE-conjugated, HPV16 E7aa49-57 peptide loaded H2-Db tetramers were obtained from the National Institute of Allergy and Infectious Diseases Tetramer Facility. Medroxyprogesterone acetate was purchased from Greenstone LLC (Peapack, NJ), and 4% Nonoxynol-9 (N-9) was purchased from Revive personal products company (Madison, NJ). Cisplatin, Tween-40 and mannitol were purchased from Sigma (St. Louis, MO).
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