Parental, NRC-03-resistant, and NRC-07-resistant MDA-MB-231 breast cancer cells were seeded at 2 × 105 cells/mL into 24-well flat-bottom tissue culture plates containing sterile coverslips and were cultured overnight to promote cell adhesion. The cells were fixed, dehydrated, dried to their critical point, mounted, and coated with gold as previously described [5 (link)]. The cells were viewed at the Institute for Research in Materials (Dalhousie University) on a Hitachi S4700 scanning electron microscope (Hitachi High Technologies, Rexdale, ON, Canada) at ×500, ×7000, and ×40,000.
S 4700 scanning electron microscope
Manufactured by Hitachi
Sourced in Japan, China
The S-4700 scanning electron microscope (SEM) is a high-performance instrument designed for advanced materials analysis. It features a field emission electron gun, which provides high-resolution imaging capabilities. The S-4700 SEM is capable of producing detailed, high-magnification images of a wide range of samples, including metals, ceramics, and biological materials.
Automatically generated - may contain errors
Lab products found in correlation
Hcp 2, by Hitachi (6 mentions)
0.05 m cacodylate buffer, by Merck Group (4 mentions)
Sodium cacodylate buffer, by Merck Group (3 mentions)
Em ace600, by Leica (2 mentions)
Co2 independent medium, by Thermo Fisher Scientific (2 mentions)
Desk 5 sputter and carbon coater, by Denton (2 mentions)
E 1030 ion sputter, by Hitachi (2 mentions)
Ht7700 transmission electron microscope, by Hitachi (2 mentions)
Jfc 1100e ion sputter, by JEOL (2 mentions)
Osmium tetroxide, by Ted Pella (1 mentions)
Pelco biowave, by Ted Pella (1 mentions)
Autosamdri 815, by Tousimis (1 mentions)
Varian 660 ir ft ir spectrometer, by Agilent Technologies (1 mentions)
K alpha model, by Thermo Fisher Scientific (1 mentions)
Cary 500, by Agilent Technologies (1 mentions)
Zen software, by Zeiss (1 mentions)
Axiophot inverted microscope, by Zeiss (1 mentions)
Autosamdri 815b, by Tousimis (1 mentions)
Av600 nmr spectrometer, by Bruker (1 mentions)
Uv 2600 spectrophotometer, by Shimadzu (1 mentions)
96 protocols using s 4700 scanning electron microscope
1
Scanning Electron Microscopy of Breast Cancer Cells
2
Characterizing OPF-DOPA Hydrogel Surface
3
SEM Preparation of Fixed Biological Samples
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Fixed samples were incubated overnight at 4 °C on cell culture treated coverslips to ensure adhesion. Next, fixed samples were rinsed with 100 mM sodium cacodylate buffer, pH 7.35 containing 130 mM sucrose. Secondary fixation was performed using 1% osmium tetroxide (Ted Pella, Inc. Redding, CA, USA) in cacodylate buffer using a Pelco Biowave (Ted Pella) operated at 100 Watts for 1 min. Specimens were next incubated at 4 °C for 1 h, then rinsed with cacodylate buffer and further with distilled water. Using the Pelco Biowave, a graded dehydration series (per exchange, 100 Watts for 40 s) was performed using ethanol. Samples were dried using the Tousimis Autosamdri 815 (Tousimis, Rockville, MD, USA) and samples were sputter coated with 5 nm of platinum using the EMS 150T-ES Sputter Coater. Images were acquired with a Hitachi S4700 scanning electron microscope (Hitachi High Technologies America, Dallas, TX, USA).
4
Accelerated Fuel Cell Degradation Test
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Square samples were prepared and weighed. A Fenton solution for an accelerated test simulating the environment of an operating PEM fuel cell [31 (link)] containing 2.5 mmol dm−3 of Fe2+ in the form of FeSO4·7H2O in 30% H2O2 was prepared. The samples were submerged in the Fenton solution for 168 h at laboratory temperature. Afterwards, they were washed with demineralized water and left to dry for 168 h at laboratory temperature. Finally, the degraded samples were weighed. An image of the surface and fracture of the samples was captured using a Hitachi S-4700 scanning electron microscope (Hitachi, Hitachi City, Japan).
5
Characterization of Matrix Composition
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The chemical characterization of matrices was performed with the use of infrared spectroscopy. FTIR spectra were recorded using Varian 660-IR FT-IR Spectrometer (Agilent, San Jose, CA, USA) in the range between 4000 and 600 cm−1 for 16 scans. Morphological characterization was performed with the use of a Hitachi S-4700 scanning electron microscope, SEM (Hitachi, Tokyo, Japan) operating at 15 kV.
6
Cochlear Ultrastructure Analysis in Cetaceans
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The right ear of SW2 was extracted and fixed in 10% neutral buffered formalin within 30 hours after death. The periotic bone surrounding the cochlea was decalcified for 9 days and 17.5 hours by using different dilutions of a specific rapid decalcifying agent RDO® (Apex Engineering Products Corporation, Aurora, Illinois, USA). Precisely, the samples were immersed in 50% RDO for the first 3 days and then transferred to 25% RDO for the remaining time. Fresh batch of the solution were prepared every 24 hours and substituted, according to a previously optimized protocol87 (link).
The decalcification of the periotic bone was stopped when the vestibular scala and the stria vascularis of the cochlea were exposed. Subsequently, the cochlea was dissected, dehydrated with increasing concentrations of ethanol, critical point dried with CO2, and then coated with gold-palladium88 (link),89 (link). The sample was evaluated with a Hitachi S-4700 scanning electron microscope (SEM) at the University of British Columbia Bioimaging Facility for evidence of acoustic trauma.
The decalcification of the periotic bone was stopped when the vestibular scala and the stria vascularis of the cochlea were exposed. Subsequently, the cochlea was dissected, dehydrated with increasing concentrations of ethanol, critical point dried with CO2, and then coated with gold-palladium88 (link),89 (link). The sample was evaluated with a Hitachi S-4700 scanning electron microscope (SEM) at the University of British Columbia Bioimaging Facility for evidence of acoustic trauma.
7
Characterization of Ag-TiO2/GF Photocatalysts
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A Hitachi S-4700 scanning electron microscope (SEM) was used to determine the surface morphologies of the Ag-TiO2/GF photocatalytic materials. X-ray diffraction (XRD) patterns were obtained using a Bruker AXN model with a Cu-Kα radiation (λ = 1.5418 Å) source operated at a scan rate of 0.02 s−1 over a 2θ range of 10–80°. A Varian Cary 500 was used to record the UV-visible diffuse reflectance spectra (DRS) of photocatalysis materials. X-ray photoelectron spectroscopy (XPS) measurements were performed using a Thermo Fisher K-alpha model to determine the chemical composition of Ag-TiO2/GF photocatalysts.
8
Scanning Electron Microscopy Sample Preparation
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Samples were fixed with 2.5% (v/v) glutaraldehyde at 4 °C for ∼24 h, washed with PBS (pH 7.2) 3 times, and postfixed in 1% (v/v) OsO4. The samples were then dehydrated through an ethanol series [30, 50, 70, 80, 90, and 100% (v/v), 3 times], critical point dried using a desiccator (HCP-2; Hitachi), and coated with gold palladium (EIKO IB-3). Images were taken with a Hitachi S-4700 scanning electron microscope (SEM) using a 2 kV accelerating voltage.
9
Shark Sperm Ultrastructure Analysis
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An aliquot of raw semen was preserved 1:10 in 0.1 M Sorenson’s phosphate buffer supplemented with 0.02% CaCl2, 0.35 M sucrose, 3.2% paraformaldehyde, and 2.5% glutaraldehyde for microscopy. Preserved sperm was stained for PMI, as described above, and imaged using a laser-scanning, confocal microscope (Zeiss 710, Thornwood, NY, USA) coupled with a Zeiss Axiophot inverted microscope in line scanning mode with a C-Apochromat 40 × Korr M27 (NA 1.2) water immersion objective, and ZEN software (Zeiss, Jena, Germany). Imaging was performed using differential interference contrast and fluorescence to highlight the boundary between the head and midpiece. Fluorescence was accomplished with laser excitation at 488 nm and 561 nm and emission collected between 500–550 nm and 575–610 nm. The length of the acrosome, head, midpiece and flagellum for 10 sperm per shark from five sharks were measured from digital photomicrographs using Fiji47 (link). For scanning electron microscopy, preserved semen was dehydrated in increasing concentrations of ethanol and critical point dried (Tousimis Autosamdri-815B, Rockville, MD). Samples were mounted onto aluminum stubs, sputter coated with Au–Pd (Leica EM ACE600, Wetzlar, Germany) and examined using a Hitachi S-4700 scanning electron microscope (SEM).
10
Scanning Electron Microscopy Sample Preparation
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