The samples were mounted on an inverted microscope (Ti-E; Nikon) equipped with a Yokogawa spinning disk head (CSU-X1) and a 512 × 512 electron multiplying charge-coupled device camera (iXON; Andor Technology). Images for each channel were collected sequentially with a 1.4 NA 100× oil immersion objective (Nikon) at room temperature. 488- and 561-nm laser illumination was used for GFP and mCherry excitation, respectively. Emission filters were 515/30 nm for GFP and 600/32 nm for mCherry.
1.4 na 100 oil immersion objective
Manufactured by Nikon
The 1.4 NA 100× oil immersion objective is a high-performance microscope objective designed for use with oil immersion techniques. It features a numerical aperture of 1.4 and a magnification of 100×, making it suitable for high-resolution imaging applications.
Automatically generated - may contain errors
3 protocols using 1.4 na 100 oil immersion objective
1
Spinning Disk Confocal Microscopy
2
Confocal Imaging of GFP, mCherry, and FM4-64
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Images for each channel were acquired simultaneously on a Nikon A1R confocal microscope system with a 1.4 NA 100× oil immersion objective (Nikon) at room temperature. 488 and 561 nm laser illumination was used for GFP/FM4-64 and mCherry excitation, respectively. Emission filters were 525/50 nm for GFP and 595/50 for mCherry/FM4-64. Image acquisition was controlled by NIS-Elements software (Nikon).
3
Spinning Disk Confocal Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The slides were mounted on an inverted microscope (model Ti-E; Nikon) equipped with a Yokogawa spinning disk head (model CSU-X1) and a 512 × 512 electron multiplying CCD camera (iXON; Andor Technology). Images for each channel were collected sequentially with a 1.4 NA 100× oil immersion objective (Nikon) at room temperature. 488 and 561 nm laser illumination was used for GFP/FM4-64 and mCherry excitation, respectively. Emission filters were 515/30 nm for GFP and 600/32 nm for mCherry/FM4-64. Image acquisition was controlled by MetaMorph software (Molecular Devices, Sunnyvale, CA).
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