GC-MS analysis of the extracts were carried out in a GC system (Agilent 7890A series, USA) equipped with split/splitless injector and auto-sampler attached to an apolar 5-MS (5% phenyl polymethyl siloxane) capillary column (Agilent 19091S-43; 30 m×0.25 mm i.d. and 0.25-μm film thickness) and fitted to Mass Detector (Agilent 5975C series, USA). The flow rate of the carrier gas, helium (He) was set to be at 1 ml.min−1 in split less mode. The injector temperature was adjusted at 250°C, while the detector temperature was fixed to 280°C. The column temperature was kept at 70°C for 1 min followed by linear programming to raise the temperature from 70° to 200°C (at 8°C min−1 with 2 min hold time), and 200°C to 250°C (at 10°C min−1 with 2 min hold time). The transfer line was heated at 280°C. Total run time was 27.2 min. Mass spectra were acquired in scan mode (70 eV); in the range of 50 to 550 m/z. Twenty microliter each of the extracts (250 mg/ml stock) were further diluted in 2 ml of methanol. One micro liter of this diluted sample was injected for GC-MS analyses.
7890a series
Manufactured by Agilent Technologies
Sourced in United States, Germany
The 7890A Series is a gas chromatograph (GC) system manufactured by Agilent Technologies. It is designed to separate, identify, and quantify chemical compounds in complex mixtures. The system features an oven, an inlet, and a detector to perform chromatographic analysis. The core function of the 7890A Series is to provide reliable and accurate gas chromatographic separation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
J w scientific hp5 ms, by Agilent Technologies (2 mentions)
Hp 5ms column, by Agilent Technologies (2 mentions)
Agilent 5975c series, by Agilent Technologies (1 mentions)
7200 gcqtof ms, by Agilent Technologies (1 mentions)
Agilent 5975c series msd, by Agilent Technologies (1 mentions)
Model no 5975c tad inert xl ei ci msd, by Agilent Technologies (1 mentions)
Hp 5ms, by Agilent Technologies (1 mentions)
Agilent 1200 series, by Agilent Technologies (1 mentions)
Acetic acid, by Maclin Biochemical Technology (1 mentions)
Propionic acid, by Maclin Biochemical Technology (1 mentions)
Butyric acid, by Maclin Biochemical Technology (1 mentions)
Quadrupole mass analyser, by Agilent Technologies (1 mentions)
5975c inert xl, by Agilent Technologies (1 mentions)
Db 5ms 5 phenyl methylpolysiloxane column, by Agilent Technologies (1 mentions)
Mat 253 mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Jmp pro v12, by SAS Institute (1 mentions)
Agilent 5977a, by Agilent Technologies (1 mentions)
Db 5 column, by Agilent Technologies (1 mentions)
Sp 2330 column, by Merck Group (1 mentions)
Db 5ms column, by Agilent Technologies (1 mentions)
24 protocols using 7890a series
1
GC-MS Analysis of Extracts
2
Quantitative Determination of α-Ketoglutarate in Serum by GC/MS
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Aplha-ketoglutarate was quantitatively determined in serum samples by GC/MS at the Centre for Omic Sciences (Rovira i Virgili University). The methodology used has been reported elsewhere [10 (link)]. Samples were analysed in a 7890A Series gas chromatograph coupled to a 7200 GCqTOF MS (Agilent Technologies, Santa Clara, U.S.A.). The chromatographic column was a J&W Scientific HP5-MS (30 m x 0.25 mm i.d., 0.25 μm film) (Agilent Technologies). The calibration curve showed good linearity in the studied range of 0.13 to 10 mg/L, with a determination coefficient (R2) of 0.9993. The extraction recovery was 99.7%, while accuracy was 101.7%. The intraday and interday precision had a relative standard deviation of, respectively, 4.1% and 4.8% (RSD, n = 3). MDL was 0.05 mg/L, while MQL was 0.13 mg/L.
3
GC-MS Analysis of Essential Oils
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Each EO sample (0.5 mL) was dissolved in 10.0 mL of GC grade n-Hexane. PTFE membrane filter paper (pore size: 0.45 μm) was used to filter the dehydrated samples. The filtered samples (1.0 μL) were injected into the GC-MS. The GC–MS analysis was performed on an Agilent 7890A series gas chromatograph interfaced to an Agilent 5975C series MSD version mass selective detector (Model No: 5975C TAD inert XL EI/CI MSD, Agilent Technologies, Palo Alto, CA, USA). Agilent 19091S–433HP-5MS 5% Phenyl Methyl Silox, 325 °C: 30 m × 250 μm x 0.25 μm column was used. The chromatography conditions were as follows (gradient): initial oven temperature: 70 °C for 4 min then increasing by 8 °C/min to 270 °C and then kept stable for 10 min, run time-39 min; carrier gas: helium at a flow rate of 1 ml/min; injection volume: 1.0 μL in split mode. The conditions of the mass unit: ion source temperature 230 °C; mass spectrum recorded at an ionization voltage of 1624 with a mass scan range of 33–550 m/z.
4
GC-qTOF Analysis of Metabolites
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Samples were analysed in a 7890A Series gas chromatograph coupled to a 7000 GC-qTOF from Agilent Technologies using the J&W Scientific HP5-MS (30 m × 0.25 mm i.d., 0.25 µm film) chromatographic column and helium as carrier gas. Ionization was done by electronic impact (EI), recording data in Full Scan mode. Quantification was performed using the corresponding analytical standard for each determined metabolite and a deuterated internal standard depending on the family of metabolite. Internal standards used were succinic acid-d4, glycerol-13C3, norvaline, L-methionine-(carboxy-13C, methyl-d3), D-glucose-13C6, myristic-d27 acid, and alpha-tocopherol-d6.
5
Evaluating Rumen Volatile Fatty Acids
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The effluent collected per day (60 mL) was used for pH and VFA assessment. These samples were collected daily before introducing a new feed and analyzed for ruminal VFA concentration (acetic, propionic and butyric acids, isobutyric acid, isovaleric acid, and valeric acid, mmol/L) from d0 to d7 using gas chromatography (Agilent, 7890A series). The lactic acid concentration was determined using high-performance liquid chromatography (Agilent 1200 series).
6
Quantification of Cecal Short-Chain Fatty Acids
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The concentrations of short‐chain fatty acids (SCFAs; acetate, propionate and butyrate) in the cecal contents were measured using gas chromatography. Acetic acid, propionic acid, and butyric acid (Macklin, Shanghai, China) were added to the fecal samples as standards. The cecal contents were diluted to 3 mL with 10 mmol/L NaOH. The fecal samples were extracted by vortexing and sonication at 0°C for 5 minutes. The pooled extract was then centrifuged (15 minutes, 10 000 rpm, 4°C). The supernatant (1 mL) was mixed with chloroform at a ratio of 1:1 to extract liposoluble components, and the aqueous phase was obtained after vortexing and sonication. Then, 0.7 mL of the aqueous phase was reacted with 1.2 µL of HCl for 10 minutes. N‐hexane (1.4 mL) was used to extract SCFAs in the aqueous phase. The extract was stored at 4°C and analyzed with an Agilent 7890A Series gas chromatograph. The capillary column was 0.25 mm × 0.25 µm × 30 m (HP‐INNOWAX). The injector temperature was maintained at 220°C, and the carrier gas was N2 at a flow rate of 1 mL min−1 in the capillary column. The column temperature was maintained at 70°C for 2 minutes. The temperature was first increased at a rate of 10°C min−1 to 150°C for 10 minutes. Then, the temperature was increased to 230°C at a rate of 15°C min−1 for 5 minutes. The running time was 30.333 minutes.
7
GC-MS Analysis of Lipid Extracts
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GC-MS was performed on both AE and TLE using a 7890A Series chromatograph attached to a 5975C Inert XL mass-selective detector with a quadrupole mass analyser (Agilent Technologies, Cheadle, UK). The carrier gas used was helium, and the inlet/column head-pressure was constant. A splitless injector was used and maintained at 300 °C. The GC column was inserted directly into the ion source of the mass spectrometer. The ionisation energy of the mass spectrometer was 70 eV and spectra were obtained by scanning between m/z 50 and 800. Two different column phases were used. General screening of both AE and TLE was performed using a DB-5 ms (5%-phenyl)-methylpolysiloxane column (30 m × 0.250 mm × 0.25 μm; J&W Scientific, Folsom, CA, USA). The temperature for this column was set at 50 °C for 2 min, then raised by 10 °C/min to 325 °C, where it was held for 15 min. The TLE was also analyzed with a HT-DB1, 100% Dimethylpolysiloxane (15 m × 0.320 mm × 0.1 µm) (J&W Scientific, Folsom, CA, USA). The injector was maintained at 350 °C. The temperature of the oven was set at 50 °C for 2 min, and then raised by 10 °C min−1 to 350 °C, where it was held for 15 min.
8
Quantifying 13CO2 Production from Labeled Chlorophyll
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13CO2 was measured in enrichment cultures containing 1.25 µmol/bottle 13C-labelled CF, 3.75 µmol/bottle non-labelled CF and 4 µM vitamin B12. The control cultures contained 5 µmol/bottle non-labelled CF and 4 µM vitamin B12. The carbon isotope composition of CO2 was determined using gas chromatography combustion isotope ratio mass spectrometry (GC/C-IRMS) consisting of a gas chromatograph (7890A Series, Agilent Technologies, Santa Clara, CA, USA) coupled via a Conflo IV interface (ThermoFinnigan, Bremen, Germany) to a MAT 253 mass spectrometer (ThermoFinnigan, Bremen, Germany). Sample separation was done with a CP-PoraBOND Q column (50 m × 0.32 mm ID, 5 µm film thickness; Agilent Technologies, Amstelveen, Netherlands) operated isothermally at 40 °C using helium as a carrier gas at a flow rate of 2 mL/min. Sample aliquots of 0.1–0.5 mL were injected at split ratios ranging from 1:10 to 1:20. The carbon isotope signatures are reported in δ notation (per mill, ‰) relative to the Vienna Pee Dee Belemnite standard.
The amount of 13CO2 produced from the 13C-labelled CF was expressed according to:
where δ13C is the 13C isotopic enrichment as compared to the standard (‰), Rsample is the 13C to 12C ratio of CO2 in the sample, and Rstandard is the international Vienna Pee Dee Belemnite standard (VPDB, 13C/12C = 0.0112372).
The amount of 13CO2 produced from the 13C-labelled CF was expressed according to:
where δ13C is the 13C isotopic enrichment as compared to the standard (‰), Rsample is the 13C to 12C ratio of CO2 in the sample, and Rstandard is the international Vienna Pee Dee Belemnite standard (VPDB, 13C/12C = 0.0112372).
9
GC-MS Analysis of Plasma Samples
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Agilent 7890A series, Germany system equipped with split-splitless injector and CTC-PAL autosampler was used for analysis of the standard and plasma samples. Apolar HP-5MS (5% phenyl polymethylsiloxane) capillary column (30 m × 0.25 mm i.d. and 0.25 μm film thickness) fitted to a mass detector was used as stationary phase. Carrier gas (Helium), used as mobile phase and the flow rate, was 1.5 mL/min for first 7 min followed by 2.5 mL/min for next 6 min, splitless. The injector temperature was maintained at 260°C, detector temperature at 300°C, while column temperature was kept at 70°C for 3 min, followed by 180°C for 3–6 min (at a rate of 35°C/min), then at 280°C for 6–8 min (at a rate of 50°C) and then kept at hold for 4 min. The transfer line was heated at 280°C. Mass spectra were acquired in electron ionization mode (70 eV) in m/z range 30–600. The peaks of the standard were identified by comparison of their mass spectra to those from NIST/NBS libraries, using different search engines. The detection limit was 1.8 µg/mL for SVP and 3.0 µg/mL for PIP.
10
GC-MS Analysis of Bioactive Essential Oils
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Analysis of the four selected bioactive EOs (O. vulgare, M. pulegium, R. officinalis, and M. communis) was performed using gas chromatography (GC) (Agilent 7890A Series) coupled to mass spectrometry (MS) equipped with a multimode injector and a 123-BD11 column of dimension (15 m × 320 μm × 0.1 μm) at Moroccan Foundation for Advanced Science, Innovation and Research (MAScIR) Institute. Ten µL of the liquid samples were dissolved in an appropriate volume of chloroform. Four µL of the soluble extract was injected into the column by 1:5 split mode using helium as carrier gas at a flow rate of 2 mL min−1. The composition of the essential oil determined from the peak areas was calculated as a percentage of the total compounds existing in the sample detection using full scan mode between 30–1000 m/z, with gain factor of 5 and electron impact ionization. The temperatures of the ion source and the quadrupoles were 230 and 150 °C, respectively. The oven temperature was programmed at 30 °C for 3 min and then increased by 10 °C min−1 to 250 °C. The compounds identification was carried out using the NIST 2017 MS Library.
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