Hen egg white lysozyme l6876

Hen egg-white lysozyme (L6876) purchased from Sigma-Aldrich, MO/USA was used for the research. It was used without further purification. Lysozyme solutions were prepared by dissolving protein powder in an aqueous NaCl solution with an ionic strength of 0.01 M or 0.15 M and pH = 7.5. The pH was established by adding small amounts of aqueous solutions of sodium hydroxide and hydrochloric acid. All other chemicals used in the study were purchased from Sigma-Aldrich, St. Louis, MO, USA. The experiments were performed at 298 K.

Hen egg white lysozyme (L6876) was obtained from Sigma-Aldrich (Saint Louis, MO, USA) and used directly. Sodium chloride (NaCl) with >99% purity was purchased from Sigma Aldrich (Saint Louis, MO, USA). Tannic acid in analytical grade was purchased from Aladdin (Shanghai, China). Hydrochloride acid (HCl) in analytical grade with a purity of 36–38% was purchased from Kemiou Chemical Reagent (Tianjin, China). Thioflavin T (ThT) was purchased from Acros (Geel, Belgium). No further purification was performed on all the chemicals. Deionized water with a resistivity of 18.2 MΩ·cm was obtained from a Millipore system (Billerica, MA, USA). Mica (muscovite) was obtained from a local vendor.