Hepes buffer solution

Pluronic^® F127, pluronic^® P105, MTX, triethylamine, methanol, acetone, dimethyl sulfoxide (DMSO), MTT and trypan blue were purchased from Sigma (St. Louis, MO, USA). Fetal bovine serum, DMEM, RPMI-1640, HEPES buffer solution, L-glutamine, gentamycin and 0.25% trypsin-EDTA were obtained from Lonza (Belgium). PIP (molecular weight of 285.34 Da and purity 98%) was provided by Alpha Aesar (Ward Hill, MA, USA).

All solvents and chemicals used in the present study have been obtained from Sigma Aldrich Co. Ltd. (St. Louis, MO, USA). L-glutamine, HEPES buffer solution, RPMI-1640, DMEM, gentamycin, 0.25% Trypsin-EDTA, and fetal bovine serum were purchased from Lonza.

Chloroform, Graphite, ethyl acetate H₂O_2, H₂SO₄, KMnO₄ NaNO₃ and NaOH were bought from Sigma (St. Louis, Mo., USA). Fetal Bovine serum, DMEM, RPMI-1640, HEPES buffer solution, L-glutamine and gentamycin were purchased from Lonza (Belgium).

Dimethyl sulfoxide (DMSO), crystal violet, and trypan blue dye were purchased from Sigma (St. Louis, MO, USA). Fetal Bovine serum, DMEM, RPMI-1640, HEPES buffer solution, L-glutamine, gentamycin, and 0.25% Trypsin-EDTA were purchased from Lonza (Basel, Switzerland). crystal violet stain (1%): It was composed of 0.5% (w/v) crystal violet and 50% methanol then made up to volume with ddH₂O and filtered through a Whatmann No.1 filter paper purchased from Sigma-Aldrich, Saint Louis, MO, USA.

The NMR spectra were recorded on Bruker Avance DRX spectrometer at 400 MHz and 500 MHz (Bruker Scientific Instruments, MA, USA) using CDCl₃ and DMSO-d₆ (Sigma, St. Louis, Mo., USA). For Column chromatography (CC), several adsorbents were used, including Polyamide SC-6 (Macherey-Nagel, Düren, Germany), silica gel G₆₀ (60–120 mesh, Merck, Darmstadt, Germany), reversed phase RP-18 silica gel (E-Merck, Darmstadt, Germany), Sephadex LH-20 (25–100 mm mesh size, E-Merck, Darmstadt, Germany) and Diaion^® HP-20 (Sorbent Technologies, Norcross, GA, USA). Pre-coated silica G₆₀ F₂₅₄ 0.25 mm and RP-18 F₂₅₄ 0.25 mm (E-Merck, Darmstadt, Germany) were used for TLC. n-hexane, dichloromethane (CH₂Cl₂), ethyl acetate (EtOAc), and methanol (MeOH) were obtained from El-Nasr Pharmaceutical and Chemical Co., Egypt. MCF-7, HepG-2, and HCT-116 cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD). Dimethyl sulfoxide, vinblastine sulfate, MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and trypan blue dye were obtained from Sigma-Aldrich Chemical Co. (St. Louis, Mo., USA). Fetal bovine serum (Lonza, Belgium). RPMI-1640 (Roswell Park Memorial Institute) medium, DMEM and HEPES buffer solution, ʟ-Glutamine, Gentamycin, Trypsin-EDTA 0.25% were obtained from Lonza Bioscience (Belgium).