Recombinant human il1 β protein

The following were purchased as indicated: (3-Aminopropyl)triethoxysilane (APTES) (Sigma Aldrich), 2-Iminothiolane hydrochloride (Traut’s reagent) (Sigma Aldrich), 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher Scientific), N-hydroxysuccinimide (NHS) (Thermo Fisher Scientific), 4-Aminothiophenol (4-ATP) (Sigma Aldrich), 2-naphthalenethiol (2-NT) (Sigma Aldrich), recombinant human TNF-α protein (TNF-α) (Abcam), Anti-TNF-α antibody (Anti-TNF-α) (Abcam), recombinant human MMP-9 protein (Abcam); Anti-MMP-9 antibody (Abcam), recombinant human IL1-α protein (Abcam), anti-IL1-α antibody (Abcam); recombinant human IL1-β protein (Abcam); rabbit polyclonal anti-IL1-β antibody (Abcam), Human MMP9 ELISA Kit - ab246539 (Abcam), bovine serum albumin (BSA) (Sigma Aldrich), ethanol (Merck) and phosphate buffered saline (PBS) (Lonza).

Recombinant human IL-1β protein (active form, 17,000 Da) was purchased from ABCAM (Cambridge, CB2 0AX, UK), together with recombinant antibodies anti- IL-1β [EPR21086], anti-IL-6 [EPR21711]. Recombinant human IL-6 protein (HumanKine) was purchased from Proteintech Group, Inc (Manchester, UK). Absolute ethanol, N-Hydroxysuccinimide (NHS), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), Lipoic acid, Ethanolamine (2-aminoethanol), PBS (dipotassium; trisodium; dihydrogen phosphate; hydrogen phosphate; dichloride) tablets, Tween 20 (2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate), human IgG and bovine serum albumin (protease- and globulin-free BSA) were purchased from Merck KGaA (Darmstadt, Germany). Human IgA were purchased from Origene Technology, Inc (Rockville, MD, USA). The cartridge for the Simple Plex Human Multianalyte Assay was from ProteinSimple (San Jose, CA, USA). The PBS solution was obtained by dissolving tablets in Milli-Q water to yield 10 mM phosphate buffer, 140 mM NaCl, and 3 mM KCl, pH 7.4. All other chemicals were diluted by using Milli-Q water or PBS solution, as reported in the text. All the reagents were at the maximum of purity and all the solutions were prepared and filtered just before the use.