Methoxy peg 2000

Chalcone MB was synthesized and characterized by the Laboratory of Organic and Pharmaceutical Chemistry, Department of Chemical Sciences, Faculty of Pharmacy/CIIMAR research group, as previously described [27 (link)]. Methoxy PEG 2000, Methoxy PEG 5000, ε-caprolactone, and Sn(oct)₂ were acquired from Sigma-Aldrich Co. (Sintra, Portugal) and methanol (HPLC grade) from VWR chemicals. IR spectra were obtained in a KBr microplate in an FTIR spectrometer Nicolet iS 10 from Thermo Scientific with the Smart OMNI-Transmission accessory (Software OMNIC 8.3, Thermo Scientific, Madison, WI, USA). ¹H and ¹³C NMR spectra were recorded in the Department of Chemistry at the University of Aveiro, Portugal, on a Bruker Avance 300 instrument (Bruker Biosciences Corporation, Billerica, MA, USA) (¹H: 300.13 MHz; ¹³C: 75.47 MHz). ¹³C NMR assignments were made in bidimensional HSQC and HMBC experiments (long-range C, H coupling constants were optimized to 7 and 1 Hz). Chemical shifts are expressed in ppm values relative to tetramethylsilane (TMS) as an internal reference and coupling constants are reported in hertz (Hz). HPLC was performed on a Dionex Ultimate 3000 (Thermo Scientific, Darmstadt, Germany).

Methoxy PEG 2000, Methoxy PEG 5000, ε-caprolactone, and Sn(oct)₂ were acquired from Sigma-Aldrich Co. (Sintra, Portugal), and methanol (HPLC grade) from VWR chemicals. 1H and 13C-NMR spectra analysis was conducted at the Department of Chemistry at the University of Aveiro, Portugal., using a Bruker Avance 300 instrument (1H: 300.13 MHz; 13C: 75.47). 13C-NMR assignments were made through bidimensional HSQC and HMBC experiments (long-range C, H-coupling constants were optimized to 7 and 1 Hz). Chemical shifts are expressed in ppm values relative to tetramethylsilane (TMS) as an internal reference, and coupling constants are reported in hertz (Hz). Cells’ reagents were purchased from Gibco (Invitrogen Corporation, Life Technologies, Renfrew, UK). Immortalized human brain capillary endothelial cells (hCMEC/D3 cell line) were kindly donated by Dr. PO Couraud (INSERM, Paris, France). Human astrocytoma U87-MG cell line was purchased from American Type Culture Collection (ATCC). For hCMEC/D3 and U-87 MG, respectively, passages 48–49 and 30–35 were used.