Diacetoxyscirpenol

All reagents
and solvents were
obtained from Fischer Scientific, Sigma-Aldrich, or TCI America and
used without further purification. Anguidine, also known as diacetoxyscirpenol
(catalog number: 34137) was purchased from Sigma-Aldrich as a diacetoxyscirpenol
solution in acetonitrile (100 μg/mL), analytical standard (HPLC
purity ≥98%). Verrucarin A (catalog number: V4877, HPLC purity
≥95%) and verrucarol (catalog number: V1628, TLC purity ≥99%)
were purchased as a powder from Sigma-Aldrich.

The following SMs were purchased: antibiotic Y (BioAustralis, Smithfield, Australia), aurofusarin (Adipogen, Epalinges, Switzerland), beauvericin (BioAustralis, Smithfield, Australia), enniatin complex A, A₁, B, B₁ (BioAustralis, Smithfield, Australia), cyclosporine (Sigma Aldrich, Brøndby, Denmark), diacetoxyscirpenol (Sigma Aldrich, Brøndby, Denmark), deoxynivalenol (Sigma Aldrich, Brøndby, Denmark), fumonisin B₁ (BioAustralis, Smithfield, Australia), fusaric acid (Sigma Aldrich, Brøndby, Denmark), moniliformin (Sigma Aldrich, Brøndby, Denmark), nivalenol (Sigma Aldrich, Brøndby, Denmark), neosolaniol (Sigma Aldrich, Brøndby, Denmark), T-2 toxin (Sigma Aldrich, Brøndby, Denmark), zearalenone (Sigma Aldrich, Brøndby, Denmark). Fusarielin A [38 (link)], fusarielin H [39 (link)] and fusarin C [40 (link)] were prepared as described in the respective publications. All metabolites were dissolved in ethanol 96% to a concentration of 51.2 mM.

Nanospheres of monodisperse polystyrene modified by carboxyl groups were acquired from Shanghai Uni Biotechnology Company Limited (SHH, CHN). We used a Millipore Milli-Q system to obtain ultrapure water. The goat anti-mouse immunoglobulin (IgG) was acquired from the Wuhan Boster Biological Technology Company Limited (WUH, HB, CHN). Europium oxide latex nanospheres, deoxynivalenol (DON), standard AFB1, diacetoxyscirpenol (DAS), ochratoxin A (OTA), fumonisin B1 (FB1), heat-stable enterotoxin (ST), T-2 toxin, zearalenone (ZEN), bovine serum albumin (BSA), disodium phosphate, boric acid, sodium chloride, Ochratoxin -A-bovine serum albumun (OTA-BSA), borax, potassium dihydrogen phosphate, methanol (MeOH), sucrose, polyvinylpyrrolidone K30 (PVPK30), acetonitrile (ACN), Tween-20 and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) were obtained from the Sigma–Aldrich Company Limited (St. Louis, MO, USA). The sample pad (GFCP000800 glass fibres, fusion three, and fusion five), absorbent pad, HFC13502 nitrocellulose (NC) membrane, and plastic adhesive card were purchased from Millipore (Billerica, MA, USA). The FF120HP NC membrane was acquired from Whatman International Ltd. (Maidstone, Kent, UK), and the CN 95 NC membrane was acquired from Sartorius (Goettingen, NDS, Germany).