The constructed plasmid pET21a-CaCDC42 containing the cdc42 gene of Candida albicans, which served as the template DNA, was recombined and prepared in the College of Life Science, Beijing Normal University, China. SYBR® Premix Ex Taq™ II containing Taq DNA polymerase catalyzing the elongation step of DNA replication, dNTPs, ROX Reference Dye II, DNA loading buffer, and SYBR Green II was purchased from Takara Bio Inc. (Dalian, China). The synthetic primers targeting cdc42 were provided by Sangon Biotech Co. Ltd. (Beijing, China) as follows: 5′-GTGATGGTGCCGTTGGTA-3′ (forward) and 5′-CCCTGTTCCTGGGTGATT-3′ (reverse), which produced a piece of DNA containing 397 bp. Thirteen heavy metal salts, ZnCl2, Pb(NO3)2, CdCl2·2.5H2O, Ni(NO3)2·6H2O, HgCl2, CrCl3, MnCl2, CuSO4, CoCl2, Ce(NO3)3, Sr(NO3)2, Bi(NO3)3 and K3Fe(CN)6 were obtained from Beijing Chemical Reagent Company (Beijing, China). Ethylene diamine tetraacetic acid (EDTA), Ethidium bromide (EB), and agarose was from Sigma-Aldrich (St. Louis, USA). The DNA replication reactions in vitro were fulfilled and monitored using the Real-time PCR System ABI7500 (Applied Biosystems™, USA).
Pb no3 2
Manufactured by Beijing Chemical Works
Sourced in China
Pb(NO3)2, also known as lead(II) nitrate, is a chemical compound that serves as a laboratory reagent. It is a crystalline solid that is soluble in water. Pb(NO3)2 has various applications in analytical chemistry, electroplating, and the production of other lead compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ni no3 2 6h2o, by Beijing Chemical Works (2 mentions)
Agarose, by Merck Group (1 mentions)
Dntps, by Takara Bio (1 mentions)
Rox reference dye 2, by Takara Bio (1 mentions)
Dna loading buffer, by Takara Bio (1 mentions)
Sybr green 2, by Takara Bio (1 mentions)
Mncl2, by Beijing Chemical Works (1 mentions)
Zncl2, by Beijing Chemical Works (1 mentions)
Hgcl2, by Beijing Chemical Works (1 mentions)
Cuso4, by Beijing Chemical Works (1 mentions)
Real time pcr system abi7500, by Thermo Fisher Scientific (1 mentions)
Cocl2, by Beijing Chemical Works (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Co no3 2 7h2o, by Beijing Chemical Works (1 mentions)
Zn no3 2 7h2o, by Beijing Chemical Works (1 mentions)
2 protocols using pb no3 2
1
Quantitative PCR of Candida CDC42 Gene
2
Synthesis of Metal-Containing Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HCl and NaOH were purchased from Kolon Chemical Co. Ltd (Chengdu, China). Ni(NO3)2·6H2O, Co(NO3)2·7H2O, Cu(NO3)2·2H2O, Zn(NO3)2·7H2O and Pb(NO3)2 were purchased from Beijing Chemical Plant (Beijing, China). 2-Acryloyl-2-methylpropionic acid (AMPS) was purchased from Aladdin Biochemical Technology Co. (Shanghai, China). Ammonium persulfate and sodium bisulfate were purchased from Windship Chemical Reagent Technology Co. (Tianjin, China). N,N′-Methylenebisacrylamide (MBA) was purchased from Maclean Biochemical Technology Co. (Shanghai, China). All aqueous solutions were prepared by deionized water.
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