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Pathscan enabler 4 histology slide scanner

Manufactured by Meyer Instruments
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The PathScan Enabler IV Histology Slide Scanner is a device designed for scanning and digitizing microscope slides. It allows for the creation of high-resolution digital images of histological samples.

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Lab products found in correlation

Tissue tek, by Sakura Finetek (1 mentions)

4 protocols using pathscan enabler 4 histology slide scanner

1

Quantitative Cell Migration Assay

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Migration assays were performed in triplicate using a 48-well micro-chemotaxis chamber. MDA-MB-231 and SUM149 cells (2.5 × 105/250 μL) were resuspended in FBS-free medium and added into each chamber. The bottom wells were filled with complete medium (500 μL) containing 10% FBS as an attractant. The cells were allowed to migrate for 6 hours and were then fixed and stained with hematoxylin and eosin. Migrated cells were scanned using the PathScan Enabler IV Histology Slide Scanner (Meyer Instruments, Inc., TX, USA) and then quantified using National Institutes of Health Image J software (http://rsb.info.nih.gov/ij/).
Bartholomeusz C., Xie X., Pitner M.K., Kondo K., Dadbin A., Lee J., Saso H., Smith P.D., Dalby K.N, & Ueno N.T. (2015). MEK inhibitor selumetinib (AZD6244; ARRY-142886) prevents lung metastasis in a triple-negative breast cancer xenograft model. Molecular cancer therapeutics, 14(12), 2773-2781.
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2

Quantifying Cell Migration and Invasion

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As described previously6 (link), migration assays were performed in triplicate using a 24-well micro-chemotaxis chamber. Invasion assays were performed using 24-well Growth Factor Reduced Corning Matrigel Invasion Chamber (Corning, NY, USA). For both assays, SUM149 and BT549 cells (1 × 105/350 μL) were resuspended in FBS-free medium and added into appropriate chambers. The bottom chamber was filled with complete medium (750 μL) containing 10% FBS as an attractant. The cells were allowed to migrate for 6 h (migration) or 24 h (invasion) and were then fixed and stained with hematoxylin and eosin. Migrated and invaded cells were scanned using the PathScan Enabler IV Histology Slide Scanner (Meyer Instruments, Inc., TX, USA) and quantified using National Institutes of Health Image J software (http://rsb.info.nih.gov/ij/).
Gagliardi M., Pitner M.K., Park J., Xie X., Saso H., Larson R.A., Sammons R.M., Chen H., Wei C., Masuda H., Chauhan G., Kondo K., Tripathy D., Ueno N.T., Dalby K.N., Debeb B.G, & Bartholomeusz C. (2020). Differential functions of ERK1 and ERK2 in lung metastasis processes in triple-negative breast cancer. Scientific Reports, 10, 8537.
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3

Cryogenic Sectioning and Scanning of Mouse Brain Tissues

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Mouse brain tissues were placed in Optimum Cutting Temperature (OCT) compound (Tissue-Tek, Sakura Finetek USA, Torrance, CA) and flash-frozen in liquid nitrogen before sectioning within a cryotome (American Optical 845 Cryo-cut Microtome, Southbridge, MA, USA) at −15°C. The brain sections were made at 12 µm thickness and placed onto polycarbonate microscope slides (P11011P; Science Supply Solutions, Elk Grove Village, IL, USA). Samples were dried in the open air, scanned at high resolution using a PathScan Enabler IV histology slide scanner (Meyer Instruments, Inc., Houston, TX, USA), and stored at −80°C before use.
Rao W., Pan N., Tian X, & Yang Z. (2016). High-Resolution Ambient MS Imaging of Negative Ions in Positive Ion Mode: Using Dicationic Reagents with the Single-Probe. Journal of the American Society for Mass Spectrometry, 27(1), 124-134.
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4

Measuring Pituitary Tumor Size in Mice

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To calculate tumor size, paraffin embedded brains were sectioned 4 microns thick in the region of pituitary tumor cell transplantation (striatum), and 3 every 15 sections were stained for hematoxilin and eosin (H&E). With this method, tumors as small as about 60 microns in length could be identified. All stained slides were scanned with PathScan Enabler IV Histology Slide Scanner (Meyer instruments) 7200 dpi, and the tumor area (pixels) was calculated with Image J (Abramoff 2004) by manually drawing a line around the edge of the tumor on each section. The largest area of each tumor was used for comparison of tumor area size.
Brain tumors derived from pituitary tumor cell transplants were identified on MRI sections as enhanced mass. Tumor volume (mm3) calculation was performed by manually drawing a line along the edge of the tumor in a magnified image of every section in which tumor was visible, then summing the tumor area (mm2) of all sections and multiplying result by section thickness (0.5mm).
Donangelo I., Ren S.G., Eigler T., Svendsen C, & Melmed S. (2014). Sca1-positive murine pituitary adenoma cells show tumor growth advantage. Endocrine-related cancer, 21(2), 203-216.
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