Tumourigenicity of MUG-Myx2a/b clones: 7 week old female nu/nu Foxn1 mice (Charles River Laboratories, Sulzfeld, Germany) were xenotransplanted with MUG-Myx2a (p49) and MUG-Myx2b (p52) cells. Respectively 5 × 106 cells were suspended in 0.2 ml of serum-free medium and subcutaneously inoculated into the right and left flank of 5 mice. The mice were observed daily and the tumour growth was monitored. All animal work was done in accordance with a protocol approved by the institutional animal care and use committee at the Austrian Federal Ministry for Science and Research (BMWF) (vote 66.010/0160-II/3b/2012).
Nu nu foxn1 mice
Manufactured by Charles River Laboratories
Sourced in Germany
The Nu/nu Foxn1 mice are a mouse model characterized by the lack of a functional Foxn1 gene, resulting in congenital athymia and a nude phenotype. These mice are commonly used in immunology and oncology research due to their impaired immune system.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using nu nu foxn1 mice
1
Xenograft Tumourigenicity Assay of MUG-Myx2 Cells
2
In Vivo Tracking of Labeled hMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell Sense-labeled hMSC at 1.5×106 and MIRB-labeled hMSC at 1.5×106 (in 100 μL HBSS/mouse) were injected IM into the right and left hind limb, respectively, in six female nude mice (nu/nu Foxn1 mice, 6–7 weeks old; Charles River Laboratories, Wilmington, MA). These injections were performed while under inhalational anesthesia (isoflurane, 1%) that lasted less than 5 minutes per mouse. For MRI, the mice were anesthetized with an intraperitoneal injection of pentobarbital (65 mg/kg) to avoid detecting isoflurane on the 19F MR images. Four of the six mice were imaged on days 0 (4–6 hours after the injection), 3, and 12 after injection. Two of the six mice were imaged on days 0, 3, 12, 17, and 26 days post-injection. A reference tube containing 2.6×1016 19F atoms/μL was always inserted into the coil with the mice.
The bSSFP parameters were the same as for the hMSC pellets but with NEX =750 and a scan time of around 1 hour. The corresponding proton images were also acquired using bSSFP, for anatomical localization of the 19F signal and to visualize the iron-labeled hMSC, with the following parameters: TR =8 milliseconds; FA =30°; rBW =125 kHz; resolution =200×200×200 μm; eight phase cycles; NEX =2; 46 minutes.
All experiments were approved by the Western University Animal Use Committee.
The bSSFP parameters were the same as for the hMSC pellets but with NEX =750 and a scan time of around 1 hour. The corresponding proton images were also acquired using bSSFP, for anatomical localization of the 19F signal and to visualize the iron-labeled hMSC, with the following parameters: TR =8 milliseconds; FA =30°; rBW =125 kHz; resolution =200×200×200 μm; eight phase cycles; NEX =2; 46 minutes.
All experiments were approved by the Western University Animal Use Committee.
3
Glioblastoma Xenograft Implantation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were carried out according to the Swiss Federal Law on the Protection of Animals, the Swiss Federal Ordinance on the Protection of Animals, and the guidelines of the Swiss confederation (permission #ZH062/15). FoxN1 nu/nu mice (Charles River, Sulzfeld, Germany) aged between 6–12 weeks were anaesthetized and placed in a stereotaxic fixation device. A burr hole was drilled in the skull 2 mm lateral and 1 mm posterior to the bregma. The needle of a Hamilton syringe was introduced into a depth of 3 mm41 . LN-229 (7.5 × 104) and LN-428 (1 × 105) cells were resuspended in PBSA and then injected into the right striatum. Animals were clinically assessed three times per week and sacrificed upon developing neurological symptoms, justifying euthanasia (score 2).
4
Intracranial Injection Pilot for MRI Detection
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!