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Anti pi 3 p

Manufactured by Echelon Biosciences
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Sourced in Germany

Anti-PI(3)P is a highly specific and sensitive antibody that recognizes phosphatidylinositol 3-phosphate (PI(3)P), a key lipid second messenger involved in a variety of cellular processes. This antibody can be used in various biochemical and cell-based assays to detect and quantify PI(3)P levels.

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Lab products found in correlation

Flag tag m2, by Merck Group (1 mentions) Atg12, by Cell Signaling Technology (1 mentions) Wipi2, by Abcam (1 mentions) Myc tag 9b11, by Cell Signaling Technology (1 mentions) Dapi stain, by Thermo Fisher Scientific (1 mentions) Anti pi 4 5 p2, by Echelon Biosciences (1 mentions)

Spelling variants of this product

Mouse anti-PI(3)P (3 citations)

2 protocols using anti pi 3 p

1

Quantification of Autophagy-related Organelles

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Cells were fixed with 4% Pfa, permeabilized with 0.2% Triton, blocked in 1% BSA and were stained with anti-PI(3)P (Echelon), LBPA (Echelon), Atg12 (Cell Signaling #2011), WIPI2 (Abcam), Flag-tag M2 (Sigma), and Myc-tag 9B11 (Cell Signaling) and imaged on upright and inverted confocal microscopes made by Zeiss (Göttingen, Germany) including Zeiss 510 and Zeiss 780. 40× and 63× oil objectives were used. For quantification, at least 30 transfected cells imaged from at least three separate experiments were examined and categorized as indicated (i.e. no rings vs. rings).
McKnight N.C., Zhong Y., Wold M.S., Gong S., Phillips G.R., Dou Z., Zhao Y., Heintz N., Zong W.X, & Yue Z. (2014). Beclin 1 Is Required for Neuron Viability and Regulates Endosome Pathways via the UVRAG-VPS34 Complex. PLoS Genetics, 10(10), e1004626.
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2

Antibody Immunostaining for Lipid Signaling

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Purified mouse monoclonal anti-PI-3,4-P2, anti-PI-3,4,5-P3, anti-PI-4,5-P2, anti-PI-3-P and polyclonal anti-hVps34 (class III PI3K) antibodies were obtained from Echelon Biosciences, Inc. (Salt Lake City, UT). Polyclonal anti-transducin alpha (Tα) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). DAPI stain used for nuclear staining and secondary antibodies were purchased from Invitrogen-Molecular Probes (Carlsbad, CA). Monoclonal anti-arrestin antibody was a kind gift from Dr. Paul Hargrave (University of Florida, Gainesville). All other reagents used for buffer preparations were of analytical grade and purchased from Sigma (St. Louis, MO).
Rajala R.V., Rajala A., Morris A.J, & Anderson R.E. (2014). Phosphoinositides: Minor Lipids Make a Major Impact on Photoreceptor Cell Functions. Scientific Reports, 4, 5463.
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