Dibasic potassium phosphate (Merck, Germany), monobasic potassium phosphate (Merck, Germany), hexadecyl trimethyl ammonium bromide (HTAB) and o-dianisidine dihydrochloride (o-dianisidine) (Sigma Chemical Co., St. Louis, Mo, USA), hydrogen peroxide (H2O2) was used for determination of myeloperoxidase (MPO) activity.
TNF-α, IL-6 and IL-1β kits (Boster, USA) were used for measurement of the cytokines.
Ketamine and xylazine vials (Alfasan, The Netherlands) were used for inducing anesthesia in rats.