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Ezh2 inhibitor gsk126

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Ezh2 inhibitor GSK126 is a laboratory reagent that can be used to inhibit the enzymatic activity of the Enhancer of zeste homolog 2 (Ezh2) protein. Ezh2 is a histone methyltransferase that plays a role in epigenetic regulation. GSK126 functions as a selective and potent Ezh2 inhibitor, allowing researchers to study the biological effects of Ezh2 inhibition in various experimental settings.

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Lab products found in correlation

Anti h3k27me3 antibody, by Cell Signaling Technology (2 mentions) S adenosylmethionine, by New England Biolabs (2 mentions) Nucleosome, by New England Biolabs (2 mentions)

2 protocols using ezh2 inhibitor gsk126

1

Chaer impact on PRC2 histone methyltransferase

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To test the impact of Chaer on PRC2 activity, we performed the histone methyltranferase activity assay as previously described66 (link). Reactions were carried out in a volume of 50 μl and contained 2 pmol nucleosome (New England Biolabs, MA, USA), 2 pmol PRC2 complex, 10 mM HEPES (pH 7.9), 0.25 mM EDTA, 200 mM NaCl, 10% glycerol, 2 mM dithiothreitol, 2.5 mM MgCl2 and 0.8 μM S-adenosyl-methionine (New England Biolabs, MA, USA) with and without 10 nM Chaer RNA or 200 nM Ezh2 inhibitor GSK126 (Merck Millipore, Darmstadt, Germany). Reactions were incubated for 2 h at 30°C, and resolved by SDS–polyacrylamide gel electrophoresis followed by immunoblotting using anti-H3K27me3 antibody (Cell Signaling Technologies, MA, USA; #9756, 1:1000).
Wang Z., Zhang X.J., Ji Y.X., Zhang P., Deng K.Q., Gong J., Ren S., Wang X., Chen I., Wang H., Gao C., Yokota T., Ang Y.S., Li S., Cass A., Vondriska T.M., Li G., Deb A., Srivastava D., Yang H.T., Xiao X., Li H, & Wang Y. (2016). A Long Non-Coding RNA Defines an Epigenetic Checkpoint in Cardiac Hypertrophy. Nature medicine, 22(10), 1131-1139.
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2

Chaer impact on PRC2 histone methyltransferase

Check if the same lab product or an alternative is used in the 5 most similar protocols
To test the impact of Chaer on PRC2 activity, we performed the histone methyltranferase activity assay as previously described66 (link). Reactions were carried out in a volume of 50 μl and contained 2 pmol nucleosome (New England Biolabs, MA, USA), 2 pmol PRC2 complex, 10 mM HEPES (pH 7.9), 0.25 mM EDTA, 200 mM NaCl, 10% glycerol, 2 mM dithiothreitol, 2.5 mM MgCl2 and 0.8 μM S-adenosyl-methionine (New England Biolabs, MA, USA) with and without 10 nM Chaer RNA or 200 nM Ezh2 inhibitor GSK126 (Merck Millipore, Darmstadt, Germany). Reactions were incubated for 2 h at 30°C, and resolved by SDS–polyacrylamide gel electrophoresis followed by immunoblotting using anti-H3K27me3 antibody (Cell Signaling Technologies, MA, USA; #9756, 1:1000).
Wang Z., Zhang X.J., Ji Y.X., Zhang P., Deng K.Q., Gong J., Ren S., Wang X., Chen I., Wang H., Gao C., Yokota T., Ang Y.S., Li S., Cass A., Vondriska T.M., Li G., Deb A., Srivastava D., Yang H.T., Xiao X., Li H, & Wang Y. (2016). A Long Non-Coding RNA Defines an Epigenetic Checkpoint in Cardiac Hypertrophy. Nature medicine, 22(10), 1131-1139.
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