Horseradish peroxidase hrp conjugated anti mouse antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Horseradish peroxidase (HRP)-conjugated anti-mouse antibody is a secondary antibody that binds to mouse primary antibodies. HRP is an enzyme that can be used as a reporter molecule in various immunoassays and detection techniques.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using horseradish peroxidase hrp conjugated anti mouse antibody

Histological Analysis of Engineered Cartilage

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples from the in vitro engineered cartilage piece and in vivo biopsied tissue were frozen in liquid nitrogen for cryosection or fixed in 4% paraformaldehyde for 24 h prior to embedding in paraffin. The samples were sectioned into 5-μm slices, mounted on glass slides, and stained with hematoxylin and eosin (HE) or Safranin-O/Fast Green (SO/FG) using previously established protocols (Zhang and Spector, 2009 ). Detection of elastin was performed using a modified Verhoeff van Gieson (EvG) elastic stain kit (Sigma-Aldrich, St. Louis, Mississippi, USA) following the manufacturer's instructions.
Expression of type II collagen was detected using mouse anti-human type II collagen monoclonal antibody (1:200 in PBS, Santa Cruz, California, USA), followed by incubation of horseradish peroxidase (HRP)-conjugated anti-mouse antibody (1:200 in PBS, Santa Cruz), and color development with diaminobenzidine tetrahydrochloride (DAB, Santa Cruz) (Yan et al., 2009 (link)).

Zhou G., Jiang H., Yin Z., Liu Y., Zhang Q., Zhang C., Pan B., Zhou J., Zhou X., Sun H., Li D., He A., Zhang Z., Zhang W., Liu W, & Cao Y. (2018). In Vitro Regeneration of Patient-specific Ear-shaped Cartilage and Its First Clinical Application for Auricular Reconstruction. EBioMedicine, 28, 287-302.

+ Open protocol

+ Expand

Evaluating EGFR-Akt-P38 Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

High glucose medium DMEM, 0.25% trypsin, PBS, penicillin-streptomycin solution, and Giemsa solution were obtained from Gibco Company. Fetal calf serum (FCS) was purchased from Sijiqing Company, Hangzhou. DMSO and Annexin V-FITC /PI cell apoptosis kit were purchased from Kaiji Company, Nanjing. Mouse monoclonal antibody against p-EGFR, p-Akt, and p-P38, mouse monoclonal antibody against β-actin, and horseradish peroxidase (HRP)-conjugated anti-mouse antibody were purchased from Santa Cruz Company. C225 (2mg/ml) was provided by the German Merck Company, Flow cytometry (FACS Calibur, Beckman Coulter Company). Linear accelerate (ELEKTA Company).

Yao Z., Peng P., Xu D., Zhou X., Pan Z., Li Z., Yao J, & Chen J. (2019). EGFR inhibitor C225 Increases the Radio-Sensitivity of Human Breast Cancer Cells. Asian Pacific Journal of Cancer Prevention : APJCP, 20(1), 311-319.

+ Open protocol

+ Expand

Cartilage Regeneration Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

To evaluate the structure and extracellular matrix (ECM) deposition of the regenerated cartilage, hematoxylin, and eosin (HE), Safranin-O, and type II collagen (mouse anti-human type II collagen monoclonal antibody, 1:100, Santa Cruz Biotechnology, Dallas, TX), staining was performed (Liu et al., 2008 (link)). The terminal deoxynucleotidyl transferase biotin dUTP nick end labeling assay (TUNEL) for apoptosis was analyzed using a TUNEL kit (Roche, Indianapolis, IN). CD3 was analyzed using mouse anti-human CD3 monoclonal antibody (1:200 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, United States). CD68 was analyzed using mouse anti-human CD68 monoclonal antibody (1:200 in PBS, Santa Cruz). Horseradish peroxidase (HRP)-conjugated anti-mouse antibody (1:200 in PBS, Santa Cruz) was then applied as a secondary antibody.

Ci Z., Zhang Y., Wang Y., Wu G., Hou M., Zhang P., Jia L., Bai B., Cao Y., Liu Y, & Zhou G. (2021). 3D Cartilage Regeneration With Certain Shape and Mechanical Strength Based on Engineered Cartilage Gel and Decalcified Bone Matrix. Frontiers in Cell and Developmental Biology, 9, 638115.

+ Open protocol

+ Expand

Western Blot and Immunohistochemistry Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used for western blot analysis and immunohistochemistry: APE/Ref-1 antibody was from Novus Biologicals (Littleton, CO, USA); AP-1/c-Fos, MMP-1, Bcl-2 and Snail antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); p84 antibody was from GeneTex Inc. (Irvine, CA, USA); α-tubulin antibody was from Sigma Life Sciences (St. Louis, MO, USA). The following secondary antibodies were used: horseradish peroxidase (HRP)-conjugated anti-mouse antibody and anti-rabbit antibody were from Santa Cruz Biotechnology.

YANG Z., YANG S., MISNER B.J., LIU-SMITH F, & MEYSKENS F.L. (2014). The role of APE/Ref-1 signaling pathway in hepatocellular carcinoma progression. International Journal of Oncology, 45(5), 1820-1828.

+ Open protocol

+ Expand

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total protein was extracted from the cells using M-PER mammalian protein extraction reagent (Pierce, IL, USA) or from tissues using T-PER tissue protein extraction reagent (Pierce). Equal amounts of protein (15 μg per lane) estimated by a bicinchoninic acid (BCA) protein assay kit (Pierce) were loaded onto (11%) SDS-PAGE gels and transferred onto nitrocellulose membranes. The blots were probed with a monoclonal antibody against human Smad4 (1:400), Smad3 (1:300), p-Smad3 (1:200), VEGF-C (1:400), and β-actin (1:1,000) (Santa Cruz, USA), followed the secondary horseradish peroxidase (HRP)-conjugated anti-mouse antibody (Santa Cruz, USA). After washing, the bands were detected by chemiluminescence and imaged with X-ray films. β-actin was used as an endogenous reference for normalization.

Li X., Lv X., Li Z., Li C., Li X., Xiao J., Liu B., Yang H, & Zhang Y. (2019). Long Noncoding RNA ASLNC07322 Functions in VEGF-C Expression Regulated by Smad4 during Colon Cancer Metastasis. Molecular Therapy. Nucleic Acids, 18, 851-862.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!