Optical rotations were obtained with a Perkin-Elmer 341 polarimeter (Boston, MA, USA). UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer, and IR was recorded on a Perkin-Elmer FT-IR 1760x spectrophotometer (Boston, MA, USA). High-resolution electrospray ionization mass spectra (HR-ESI-MS) were recorded with a Bruker micro TOF mass spectrometer (Billerica, MA, USA). NMR spectra were obtained with a Bruker Avance DPX-300 FT-NMR spectrometer (Billerica, MA, USA). Vacuum liquid chromatography (VLC) and column chromatography (CC) were performed on silica gel 60 (Merck, 70–230 µm, Darmstadt, Germany), silica gel 60 (Merck, 230–400 nm) or Sephadex LH-20 (Pharmacia, Piscataway, NJ, USA). Yeast α-glucosidase enzyme, p-nitrophenol-α-d -glucopyranoside, pancreatic lipase, and 4-methylumbelliferyl oleate were purchased from Sigma Chemical, Inc. (St. Louis, MO, USA), and acarbose from Fluka Chemical (Buchs, Switzerland).
Ft ir 1760x spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The FT-IR 1760X spectrophotometer is a laboratory instrument designed for Fourier Transform Infrared (FT-IR) spectroscopy. It is capable of measuring the infrared absorption spectrum of samples, providing information about their molecular composition and structure.
Automatically generated - may contain errors
Lab products found in correlation
Microtof mass spectrometer, by Bruker (8 mentions)
Avance dpx 300 ft nmr spectrometer, by Bruker (6 mentions)
Avance 3 hd 500 nmr spectrometer, by Bruker (5 mentions)
Kieselgel 60, by Merck Group (4 mentions)
341 polarimeter, by PerkinElmer (4 mentions)
Spectronic 300 array spectrophotometer, by PerkinElmer (3 mentions)
Avance neo 400 mhz nmr spectrometer, by Bruker (3 mentions)
Yeast α glucosidase enzyme, by Merck Group (2 mentions)
P nitrophenol α d glucopyranoside, by Merck Group (2 mentions)
Silica gel 60, by Merck Group (2 mentions)
Spectronic 3000 array spectrophotometer, by Milton Roy (2 mentions)
Spectronic 3000 array spectrophotometer, by PerkinElmer (2 mentions)
Sephadex lh 20, by GE Healthcare (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Pancreatic lipase, by Merck Group (1 mentions)
4 methylumbelliferyl oleate, by Merck Group (1 mentions)
Clariostar apparatus, by BMG Labtech (1 mentions)
Etoac, by Merck Group (1 mentions)
Ch2cl2, by Merck Group (1 mentions)
N butanol, by Merck Group (1 mentions)
11 protocols using ft ir 1760x spectrophotometer
1
Spectroscopic Analysis and Enzyme Assays
2
Spectroscopic Characterization of Compounds
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Optical rotations were determined with a PerkinElmer Polarimeter 341 (Boston, MA, USA). UV spectra were recorded on a Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were obtained with a PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were measured using a Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded on a Bruker Avance DPX-300FT NMR spectrometer or a Bruker Avance III HD 500 NMR spectrometer (Billerica, MA, USA). Yeast α-glucosidase enzyme and p-nitrophenol-α-d -glucopyranoside were obtained from Sigma Chemical, Inc. (St. Louis, MO, USA), and acarbose was purchased from Fluka Chemical (Buchs, Switzerland). Microtiter plate readings were carried out with a CLARIOstar apparatus (BMGLABTECH, Ortenberg, Germany).
3
Spectroscopic Characterization of Novel Compounds
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UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer, and the IR was recorded on a Perkin-Elmer FT-IR 1760x spectrophotometer (Boston, MA, USA). High-resolution electrospray ionization mass spectra (HR-ESI-MS) were recorded with a Bruker micro TOF mass spectrometer (Billerica, MA, USA). NMR spectra were obtained with a Bruker Avance DPX-300 or Avance III HD 500FT-NMR spectrometer (Billerica, MA, USA). MeOH, EtOAc, n-butanol, hexane, CH2Cl2, yeast α-glucosidase, p-NPG, glucose oxidase (GO) assay kit, sodium dodecyl sulfate (SDS), 3-(4,5- dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide (MTT), and acarbose were obtained from Sigma-Aldrich (St. Louis, MO, USA). Alpha minimal essentialmedium (α-MEM), fetal bovine serum (FBS), and penicillin-streptomycin (10,000 IU/mL) were purchased from Thermo Fisher Scientific (Grand Island, NY, USA). Insulin (100 IU/mL) was acquired from Biocon (Bangalore, India). Silica gel (SiO2) and TLC plates were obtained from Merck (Darmstadt, Germany), and Sephadex LH-20 was from Pharmacia (Piscataway, NJ, USA).
4
Spectroscopic Characterization of Cellular Oxidative Stress
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The Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA) was used to record UV spectra, while IR spectra were obtained from the PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were determined using the Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded by the Bruker Avance DPX-300FT NMR spectrometer or the Bruker Avance III HD 500 NMR spectrometer (Billerica, MA, USA). Sigma Aldrich (Sigma-Aldrich, Dorset, UK) provided 2′,7′-dichlorofluorescein di-acetate (DCFH-DA), and Merck Millipore (Merck Millipore, Darmstadt, Germany) supplied fetal bovine serum (FBS). Invitrogen (Invitrogen Ltd., Paisley, UK) provided Dulbecco’s modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F-12), penicillin–streptomycin, H2O2, dimethyl sulfoxide (DMSO), and 3-[4,5-dimethyltiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT). Antibodies used in western blotting were obtained from Cell Signaling Technology (Danvers, MA, USA). Antioxidant enzyme assay test kits were obtained from Cayman Chemical (Cayman Chemical, Ann Arbor, MI, USA).
5
Spectroscopic and Chromatographic Techniques
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UV spectra were recorded on a Milton Roy Spectronic 300 Array spectrophotometer (Rochester, Monroe, NY, USA), and IR spectra were obtained on a Perkin-Elmer FT-IR 1760X spectrophotometer (Norwalk, Fairfield, CT, USA). Optical rotation was measured on a Perkin-Elmer 341 polarimeter (Norwalk, Fairfield, CT, USA). Mass spectra were recorded on a Bruker micro TOF mass spectrometer (ESI-QqTOF-MS) (Manchester, UK). NMR spectra were recorded on a Bruker Avance DPX-300 FT-NMR spectrometer or a Bruker Avance III HD 500 NMR spectrometer (Rheinstetten, Germany). Column chromatography (CC) was performed on silica gel 60 (Kieselgel 60, 70–320 µm, Merck, Darmstadt, Germany), silica gel 60 (Kieselgel 60, 230–400 µm, Merck, Darmstadt, Germany), C-18 (Kieselgel 60 RP-18, 40–63 µm, Merck, Darmstadt, Germany), Diaion HP (Mitsubishi Chemical, Tokyo, Japan) and Sephadex LH-20 (25–100 µm, GE Healthcare, Göteborg, Sweden).
6
Analytical Techniques for Compound Characterization
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Optical rotation was
determined by a Jasco P-2000 digital polarimeter (Easton, MD, USA).
UV spectra were recorded with a Milton Roy Spectronic 3000 Array spectrophotometer
(Rochester, Monroe, NY, USA). CD spectra were obtained from a Jasco
J-815 CD spectrophotometer (Hachioji, Tokyo, Japan). FT-IR spectra
were measured with a PerkinElmer FT-IR 1760X spectrophotometer (Boston,
MA, USA). The Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica,
MA, USA) was used to measure mass spectra. NMR spectra were carried
through a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA,
USA). Semipreparative high-performance liquid chromatography (HPLC)
was conducted using the Shimadzu HPLC (Kyoto, Japan). Chromatography
was conducted by silica gel 60 (no. 1.07734.2500), size 0.063–0.200
mm and (no. 1.09385.2500), size 0.040–0.063 mm (Merck, NJ,
USA). Reverse-phase column chromatography was done by RP-18 (no. 1.13900.1000),
size 40–63 μm (Merck KGaA, Darmstadt, Germany). Sephadex
LH-20 (Merck, NJ, USA) was also used for the isolation of compounds.
Diaion HP-20 column chromatography was performed on polar copolymer
styrene-divinylbenzene adsorbent resin with a particle size of 0.5
mm in diameter (Mitsubishi, Tokyo, Japan). The purity of isolated
compounds was initially assessed through thin-layer chromatography,
silica gel 60 F254 plates (Merck, NJ, USA) under UV light.
determined by a Jasco P-2000 digital polarimeter (Easton, MD, USA).
UV spectra were recorded with a Milton Roy Spectronic 3000 Array spectrophotometer
(Rochester, Monroe, NY, USA). CD spectra were obtained from a Jasco
J-815 CD spectrophotometer (Hachioji, Tokyo, Japan). FT-IR spectra
were measured with a PerkinElmer FT-IR 1760X spectrophotometer (Boston,
MA, USA). The Bruker MicroTOF mass spectrometer (ESI-MS) (Billerica,
MA, USA) was used to measure mass spectra. NMR spectra were carried
through a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA,
USA). Semipreparative high-performance liquid chromatography (HPLC)
was conducted using the Shimadzu HPLC (Kyoto, Japan). Chromatography
was conducted by silica gel 60 (no. 1.07734.2500), size 0.063–0.200
mm and (no. 1.09385.2500), size 0.040–0.063 mm (Merck, NJ,
USA). Reverse-phase column chromatography was done by RP-18 (no. 1.13900.1000),
size 40–63 μm (Merck KGaA, Darmstadt, Germany). Sephadex
LH-20 (Merck, NJ, USA) was also used for the isolation of compounds.
Diaion HP-20 column chromatography was performed on polar copolymer
styrene-divinylbenzene adsorbent resin with a particle size of 0.5
mm in diameter (Mitsubishi, Tokyo, Japan). The purity of isolated
compounds was initially assessed through thin-layer chromatography,
silica gel 60 F254 plates (Merck, NJ, USA) under UV light.
7
Comprehensive Analytical Characterization
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NMR spectra were obtained using a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA, USA). Mass spectra were recorded on a Bruker micro TOF mass spectrometer (ESI-TOF-MS) (Billerica, MA, USA). Optical rotation was obtained using Jasco p-2000 digital polarimeter (Easton, MD, USA). The ECD spectra were calculated by JASCO J-810. UV spectra were measured using a Milton Roy Spectronic 3000 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were determined with a PerkinElmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Semi-preparative HPLC was performed using the Shimadzu HPLC (Kyoto, Japan).
8
Analytical Techniques for Compound Characterization
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Bruker micro TOF mass spectrometer (ESI-MS) was used for mass spectrometric analysis and a Milton Roy Spectronic 300 Array spectrophotometer was utilized for UV spectroscopic measurement. NMR spectra were obtained on a Bruker Avance III HD 500 NMR spectrometer or a Bruker Avance DPX-300 FT-NMR spectrometer. The recording of IR spectra was done by a Perkin-Elmer FT-IR 1760X spectrophotometer. Column chromatography (CC) was conducted on different stationary phases. These were silica gel 60 (Merck, Kieselgel 60, 70–320 μm), silica gel 60 (Merck, Kieselgel 60, 230–400 μm), C-18 (Merck, Kieselgel 60 RP-18, 40–63 μm) and Sephadex LH-20 (25–100 μm, GE Healthcare).
9
Characterization of Organic Compounds
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UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer (Rochester, Monroe, NY, USA). IR spectra were recorded on a Perkin-Elmer FT-IR 1760X spectrophotometer (Boston, MA, USA). Mass spectra were obtained on a Bruker micro TOF mass spectrometer (ESI-MS) (Billerica, MA, USA). NMR spectra were recorded on a Bruker Avance Neo 400 MHz NMR spectrometer (Billerica, MA, USA). Vacuum liquid column chromatography (VLC) and column chromatography (CC) were performed on silica gel 60 (Merck, Kieselgel 60, 70–320 µm) and silica gel 60 (Merck, Kieselgel 60, 230–400 µm) (Darmstadt, Germany).
10
Spectroscopic Characterization of Compounds
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UV spectroscopic data were determined using an Agilent Cary 60 Spectrophotometer (Penang, PG, Malaysia), and the IR data were obtained via a Perkin–Elmer FT-IR 1760x spectrophotometer (Boston, MA, USA). High-resolution Atmospheric Pressure Chemical Ionization mass spectra (HR-APCI-MS) were recorded with a Bruker micro TOF-QII mass spectrometer (Billerica, MA, USA). 1H and 13C NMR spectra were recorded with a Bruker Avance DPX-300 (Billerica, MA, USA).
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