Recombinant human adamts 4

Manufactured by R&D Systems

Sourced in United States

Recombinant human ADAMTS-4 is a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family protein produced in a mammalian cell expression system. ADAMTS-4 is a secreted enzyme that cleaves aggrecan, a major component of the extracellular matrix in cartilage.

Automatically generated - may contain errors

Lab products found in correlation

Batimastat, by Merck Group (1 mentions) Vt1000, by Leica (1 mentions) Mmp 13, by R&D Systems (1 mentions)

3 protocols using recombinant human adamts 4

In vitro PARP-1 Proteolysis by ADAMTS-4

Check if the same lab product or an alternative is used in the 5 most similar protocols

For in vitro proteolysis studies, 0.5 μg recombinant human PARP-1 (Life Technologies, Carlsbad, CA, USA) was incubated with 0.44 μg affinity-purified, recombinant human ADAMTS-4 (R&D Systems, Minneapolis, MN, USA) in reaction buffer (50 mM Tris, 10 mM CaCl₂, pH 7.5) with a total volume of 25 μl at 37 °C for 1 to 3 hours. The broad-spectrum pan extracellular matrix protease inhibitor batimastat (Sigma-Aldrich; 0.125 mg/ml) was used to inhibit ADAMTS-4 activity. The products of PARP-1 digestion were analysed by western blot.

Ren P., Hughes M., Krishnamoorthy S., Zou S., Zhang L., Wu D., Zhang C., Curci J.A., Coselli J.S., Milewicz D.M., LeMaire S.A, & Shen Y.H. (2017). Critical Role of ADAMTS-4 in the Development of Sporadic Aortic Aneurysm and Dissection in Mice. Scientific Reports, 7, 12351.

+ Open protocol

+ Expand

Cartilage Explant Culture with ADAMTS-4 and MMP-13

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human ADAMTS-4 (R&D Systems, Minneapolis, MN, USA) and MMP-13 (R&D Systems, Minneapolis, MN, USA) were diluted to 50 ng/ml in the tissue explant culture medium. Cartilage explant tissues were incubated in the control culture medium and 50 ng/ml ADAMTS-4 at 37 °C and 5% CO₂ for 7 days; or incubated in the control culture medium and 50 ng/ml MMP-13 at 37 °C and 5% CO₂ for 1, 4 or 7 days respectively. The plugs were then sliced perpendicular to the articular surface in 30 μm-thick sections by a vibratome (VT-1000S, Leica Microsystems Inc., Germany) in physiological buffer to preserve cell viability. Cartilage sections were washed thoroughly with PBS and pre-stained with Calcein AM and DAPI (Life Technologies, Grand Island, NY, USA) to identify live cells, and then affixed to a coverslip by applying a small drop of cyanoacrylate (Loctite, Westlake, OH, USA) to each end of the tissue sections but not the AFM testing regions as previously described^{23 (link)}.

McCreery K.P., Xu X., Scott A.K., Fajrial A.K., Calve S., Ding X, & Neu C.P. (2021). Nuclear stiffness decreases with disruption of the extracellular matrix in living tissues. Small (Weinheim an der Bergstrasse, Germany), 17(6), e2006699.

+ Open protocol

+ Expand

Quantifying ADAMTS4 in Mouse Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

The blood samples (0.4 ml) from the C57Bl/6J wild type, ApoE^−/− and ApoE^−/−Adamts4^−/− knockout mice were drawn through cardiac puncture into a tube containing 0.1 ml EDTA (0.5 M). The samples were centrifuged at 2,500 rpm for 10 min and the supernatant was carefully transferred and used for ADAMTS4 quantification using an indirect ELISA method. Recombinant human ADAMTS4 (R&D Systems, USA) was used to plot the standard curve. Anti-human ADAMTS4 antibody had a cross reactivity for mouse ADAMTS4 recognizing the identical epitope present in both human and mouse (MAHVDPEEP).

Kumar S., Chen M., Li Y., Wong F.H., Thiam C.W., Hossain M.Z., Poh K.K., Hirohata S., Ogawa H., Angeli V, & Ge R. (2016). Loss of ADAMTS4 reduces high fat diet-induced atherosclerosis and enhances plaque stability in ApoE−/− mice. Scientific Reports, 6, 31130.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!