Rd291108200r

Manufactured by BioVendor

Sourced in Czechia

The RD291108200R is a laboratory equipment product manufactured by BioVendor. It is designed for use in scientific research and analysis. The core function of this product is to provide a reliable and precise measurement tool for researchers and scientists.

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Lab products found in correlation

Mouse and rat fgf 21 elisa, by BioVendor (3 mentions) Biotek synergy h1, by Agilent Technologies (2 mentions) Accutrend technology, by Roche (2 mentions) 80 insmsu e01, by ALPCO (1 mentions) Human fgf21 elisa, by BioVendor (1 mentions) Spectramax m2, by Molecular Devices (1 mentions) Madkmag hk, by Merck Group (1 mentions)

11 protocols using rd291108200r

Quantification of FGF21 and Insulin in Plasma

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Concentrations of mouse and recombinant human FGF21 in plasma were determined by specific ELISAs according to the procedure recommended by the manufacturer (no. RD291108200R, mouse and rat FGF-21 ELISA; no. RD191108200R, human FGF21 ELISA; BioVendor, Brno, Czech Republic) and as described previously [3 (link), 4 (link)]. Plasma insulin concentrations were determined with an ELISA (80-INSMSU-E01; ALPCO Diagnostics, Salem, NH, USA) [37 (link)].

Laeger T., Baumeier C., Wilhelmi I., Würfel J., Kamitz A, & Schürmann A. (2017). FGF21 improves glucose homeostasis in an obese diabetes-prone mouse model independent of body fat changes. Diabetologia, 60(11), 2274-2284.

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Quantifying Serum FGF21 Levels

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Serum concentrations of FGF21 were determined using a commercially available colorimetric sandwich ELISA (RD291108200R, BioVendor, Brno, Czech Republic) according to the manufacturer's instructions. Serum samples were diluted 10–20 times prior to analysis. The resulting absorbance was measured on a microplate reader (BioTek Synergy H1, Agilent Technologies) and FGF21 concentrations were calculated using the four‐parameter approach in R²⁶ using RStudio²⁷ and the drc package.²⁸ For samples with concentrations below the limit of detection, we computed their concentration as equal to the lowest detected.

Jonsson W.O., Mirek E.T., Wek R.C, & Anthony T.G. (2022). Activation and execution of the hepatic integrated stress response by dietary essential amino acid deprivation is amino acid specific. The FASEB Journal, 36(7), e22396.

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Quantifying Serum FGF21 Levels

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Serum concentrations of FGF21 were determined using a commercially available colorimetric sandwich ELISA (RD291108200R, BioVendor, Brno, Czech Republic) according to the manufacturer’s instructions. Serum samples were diluted 10–20 times prior to analysis. The resulting absorbance was measured on a microplate reader (BioTek Synergy H1, Agilent Technologies) and FGF21 concentrations were calculated using the four-parameter approach in R (26 ) using RStudio (27 ) and the drc package (28 ). For samples with concentrations below the limit of detection, we computed their concentration as equal to the lowest detected.

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Quantifying Serum FGF21 Levels in Mice

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Concentrations of FGF21 in serum were determined in mice with an ELISA according to the procedure recommended by the manufacturer (no. RD291108200R, Mouse and Rat FGF-21 ELISA, BioVendor). The minimal detectable concentration of FGF21 with this assay was 18.4 pg/ml. For determination of serum FGF21, 50 µl of serum were diluted in 200 μl of dilution buffer before analysis.

Hill C.M., Laeger T., Albarado D.C., McDougal D.H., Berthoud H.R., Münzberg H, & Morrison C.D. (2017). Low protein-induced increases in FGF21 drive UCP1-dependent metabolic but not thermoregulatory endpoints. Scientific Reports, 7, 8209.

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FGF21 ELISA in Mouse Serum

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Fibroblast Growth Factor 21 Mouse (FGF21) ELISA was obtained from Biovendor RD291108200R (Czech Republic), and performed using serum from CD and HFD-fed mice, Scr vs. Mafg LNA treated following the manufacturer’s instructions.

Pradas-Juni M., Hansmeier N.R., Link J.C., Schmidt E., Larsen B.D., Klemm P., Meola N., Topel H., Loureiro R., Dhaouadi I., Kiefer C.A., Schwarzer R., Khani S., Oliverio M., Awazawa M., Frommolt P., Heeren J., Scheja L., Heine M., Dieterich C., Büning H., Yang L., Cao H., Jesus D.F., Kulkarni R.N., Zevnik B., Tröder S.E., Knippschild U., Edwards P.A., Lee R.G., Yamamoto M., Ulitsky I., Fernandez-Rebollo E., Vallim T.Q, & Kornfeld J.W. (2020). A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism. Nature Communications, 11, 644.

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Quantifying Serum FGF21 Levels

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Serum concentrations of FGF21 were measured using sandwich ELISA, according to the manufacturer's instructions (RD291108200R; BioVendor LLC). Samples were assayed in duplicate on a 96-well plate and the absorbance measured spectrophotometrically (SpectraMax M2; Molecular Devices LLC) at 450 nm, with 630 nm as the reference wavelength. The average coefficient of variation for technical duplicates in this analysis was ∼12.7%. Concentrations were derived using the resulting 4-parameter logistic standard curve, constructed using the “dcr” package in R (36 , 37 (link)).

Jonsson W.O., Margolies N.S., Mirek E.T., Zhang Q., Linden M.A., Hill C.M., Link C., Bithi N., Zalma B., Levy J.L., Pettit A.P., Miller J.W., Hine C., Morrison C.D., Gettys T.W., Miller B.F., Hamilton K.L., Wek R.C, & Anthony T.G. (2021). Physiologic Responses to Dietary Sulfur Amino Acid Restriction in Mice Are Influenced by Atf4 Status and Biological Sex. The Journal of Nutrition, 151(4), 785-799.

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Quantifying Serum FGF21 in Mice

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Concentrations of FGF21 in serum were determined in mice with an ELISA according to the procedure recommended by the manufacturer (no. RD291108200R, Mouse and Rat FGF-21 ELISA, BioVendor). The minimal detectable concentration of FGF21 with this assay was 18.4 pg/ml. The intra- and inter-assay coefficients of variation for the mouse and rat FGF21 assay were both < 9.0%, respectively. For determination of serum FGF21, 50 μl of serum were diluted in 200 μl of dilution buffer before analysis.

Laeger T., Albarado D.C., Burke S.J., Trosclair L., Hedgepeth J.W., Berthoud H.R., Gettys T.W., Collier J.J., Münzberg H, & Morrison C.D. (2016). Metabolic responses to dietary protein restriction require an increase in FGF21 that is delayed by the absence of GCN2. Cell reports, 16(3), 707-716.

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FGF21 Plasma Levels in Diabetic Mice

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FGF21 plasma levels were quantified by ELISA (RD291108200R, BioVendor) following the manufacturer's instructions. Samples were collected from the db+ and db/db mice treated with resveratrol or not for 8 weeks and after 4 h of fasting.

Escalona-Garrido C., Vázquez P., Mera P., Zagmutt S., García-Casarrubios E., Montero-Pedrazuela A., Rey-Stolle F., Guadaño-Ferraz A., Rupérez F.J., Serra D., Herrero L., Obregon M.J, & Valverde Á.M. (2020). Moderate SIRT1 overexpression protects against brown adipose tissue inflammation. Molecular Metabolism, 42, 101097.

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Metabolic Biomarker Quantification

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Glucose and triglyceride levels in blood were measured using Accutrend Technology (Roche Diagnostics). Plasma FGF21 was quantified using an enzyme-linked immunosorbent assay (ELISA) (RD291108200R, BioVendor). Plasma insulin and leptin levels were quantified using a Multiplex system (MADKMAG‐HK, Merck Millipore).

Delgado-Anglés A., Blasco-Roset A., Godoy-Nieto F.J., Cairó M., Villarroya F., Giralt M, & Villarroya J. (2023). Parkin depletion prevents the age-related alterations in the FGF21 system and the decline in white adipose tissue thermogenic function in mice. Journal of Physiology and Biochemistry, 80(1), 41-51.

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FGF21 Serum Concentration Quantification

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Serum concentrations of FGF21 were measured using a commercially available sandwich enzyme-linked immunosorbent assay (ELISA; RD291108200R; BioVendor LLC). The ELISA assay was performed according to the manufacturer’s instructions as previously reported (18 (link)).

Levy J.L., Mirek E.T., Rodriguez E.M., Zalma B., Burns J., Jonsson W.O., Sampath H., Staschke K.A., Wek R.C, & Anthony T.G. (2023). GCN2 is required to maintain core body temperature in mice during acute cold. American Journal of Physiology - Endocrinology and Metabolism, 325(5), E624-E637.

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