Ha 2367

PHF1 (phospho-Ser396/404) and 12E8 (phospho-Ser262) antibodies were kindly provided by Dr. P. Davies and Dr. P. Seubert, respectively. Anti-tau polyclonal (A0024) antibody was purchased from Dako. Anti-Nrf2 (12721), TFEB (4220), mTOR (2983), pmTOR (phospho-Ser2448, 5536), p70S6K (2708), pp70S6K (phospho-Thr389, 9234), 4E-BP1 (9644), p4E-BP1 (phospho-Thr37/46, 2855), NDP52 (9036) and HA (2367) antibodies were purchased from Cell Signaling Technology. Anti-LC3 antibody (PD014) was obtained from Medical & Biological Laboratories. Anti-lamin (A/C) (SC-6215) and β-actin (MAB1501) antibodies were purchased from Santa Cruz Biotechnology and Millipore, respectively. Anti-heme oxygenase (HO)-1 (ADI-SPA-895) and p62/SQSTM1 (BML-PW9860) antibodies were obtained from Enzo Life Sciences. Anti-ATG9b (PA5-20998) antibody was purchased from Thermo Fisher Scientific. Anti-tubulin (T6074) antibody was obtained from Sigma. The GFP-LC3 plasmid was used previously18 (link). The GSK-3β-S9A plasmid was kindly provided by Dr. E. Choi. The mouse TFEB specific predesigned siRNA (S74859) was purchased from Life Technology. The ON-TARGET plus mouse Nrf2 (18024) siRNA SMART pool was purchased from GE Healthcare. Fisetin (5016) was purchased from Tocris. Chemicals such as bafilomycin A1 (B1793) and chloroquine (C6628) were obtained from Sigma.

The human p-CMV-ICAM-1 vector was purchased from sinobiological, pc-DNA3-SRC vector was purchased from Addgene, and p-CMV6-c-MET vector was purchased from origene. HGF (100-39H) was purchased from Peprotech. SU11274 (c-MET inhibitor), WP1066 (573097), and PP2 (529573) were obtained from Calbiochem. Antibodies specific for CDH2 (#610920) was purchased from BD Biosciences (San Jose, CA, USA). Antibodies specific for Snail(#3879), Slug (#9585), TWIST (#46702),SRC (#2108), p-SRC (#2105), MET (#3127), p-MET (#3077), and HA (#2367) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies specific for Vim (ab137321), CDH1 (ab1416), PDGFB (ab23914), VEGFA (ab46154), FGF2 (ab92337), STAT3 (ab226942), p-STAT3 (ab32143), ZEB1 (ab223688) and ICAM-1 (ab2213) was obtained from Abcam (Cambridge, UK). The monoclonal antibodies specific for β-Actin (SC-47778) was purchased from Santa Cruz. The monoclonal antibodies specific for FLAG (F-3165) was purchased from Sigma. The polyclonal antibodies specific for p-ICAM-1 (CSB-PA000547) was purchased from CUSABIO TECHNOLOGY.