Marchantin M was isolated from Chinese Liverworts17 (link). It was dissolved in dimethyl sulfoxide (DMSO) and stored at −20 °C. Lipopolysaccharide (LPS) was purchased from Sigma–Aldrich (St. Louis, MO, USA). Thapsigargin (Tg) was purchased from Selleck (Houston, TX, USA). Doxorubicin (Doxo) and docetaxel (Doc) were purchased from the Second Hospital of Shandong University (Jinan, China).
Thapsigargin tg
Manufactured by Selleck Chemicals
Sourced in United States, China
Thapsigargin (Tg) is a potent selective inhibitor of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) enzyme. It functions by blocking the transport of calcium ions (Ca2+) out of the cell, leading to an increase in intracellular calcium concentration.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharide lps, by Merck Group (1 mentions)
Recombinant human il 6, by R&D Systems (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Mkc 8866, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Omega Scientific (1 mentions)
Du145, by Shanghai Cell Bank (1 mentions)
2 protocols using thapsigargin tg
1
Extraction and Storage of Marchantin M from Liverworts
2
Prostate Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human prostate cancer cell lines LNCaP, VCaP, PC3, and C4-2B were purchased from the American Type Culture Collection. DU145 and LAPC4 cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). PC-3, VCaP, and LNCaP cells were cultured in RPMI-1640 medium containing 10% FBS. Dulbecco’s Modified Eagle Medium (DMEM) was used as the culture medium for prostate cancer cells of DU-145, C4-2B, and LAPC4. All cell lines have recently been authenticated by using STR-analysis. Fetal bovine serum (FBS) and charcoal-stripped fetal bovine serum (CSS) were purchased from Omega Scientific (Tarzana, CA). Recombinant human IL-6 and neutralizing anti-human IL-6 antibody were purchased from R&D Systems Inc. (Minneapolis, MN, USA). MKC8866 and thapsigargin (TG) were purchased from Selleck Chemicals (Shanghai, China).
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