Taz flox

Mice. The Nf1-KO (60 (link)), Nf1 flox (61 (link)), Hoxb7-Cre (62 (link)), Lats1 flox (63 (link)), Lats2 flox (63 (link)), Yap flox (64 (link)), Taz flox (65 (link)), and athymic nude mice (Foxn1^–/–) were purchased from Jackson Laboratory. Genotyping was performed by PCR as previously reported (60 (link)–65 (link)). All mice were housed in the Animal Care Facility at the UT Southwestern Medical Center (Dallas, Texas, USA).

Animal treatment and care followed NIH guidelines and the requirements of Carnegie Institution, and approved by Carnegie Institutional Animal Care and Use Committee. Mpp7^flox mice was generated via contractual service with ALSTEM Inc., and available upon request. Pax7^CreERT2 mice (Lepper et al., 2009 (link)) were donated by Dr. C Lepper. Amot^flox mice (Shimono and Behringer, 2003 (link)) was obtained from Dr. J Kissil. Yap^flox, Taz^flox (Reginensi et al., 2013 (link)), and Rosa26^YFP (Srinivas et al., 2001 (link)) mice were obtained from The Jackson Laboratory. Mice were genotyped by PCR using tail DNA by allele-specific oligonucleotides (information available upon request). Appropriate mating schemes were performed to obtain control and experimental mice stated in text, figures and legends. Both male and female mice were used and included in data analysis unless specified otherwise.