Bio rex 70

Manufactured by Bio-Rad

Sourced in United States

Bio-Rex 70 is a strong cation exchange resin used for the separation and purification of biomolecules. It is made of sulfonated polystyrene resin and has a high exchange capacity. Bio-Rex 70 can be used in a variety of applications, including protein purification, ion exchange chromatography, and buffer exchange.

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Lab products found in correlation

Bio gel p 2, by Bio-Rad (3 mentions) Cy5 maleimide monoreactive dye, by GE Healthcare (1 mentions) Hispur ni nta, by Thermo Fisher Scientific (1 mentions) Monoq 4.6 100 pe, by GE Healthcare (1 mentions) Activated charcoal, by Fujifilm (1 mentions) Crystalline ttx, by Fujifilm (1 mentions) Hitrap q, by GE Healthcare (1 mentions) Hitrap q hp, by GE Healthcare (1 mentions) Superdex 200, by GE Healthcare (1 mentions)

Close spelling variants of this product

Bio-Rex 70 cation-exchange resin

6 protocols using bio rex 70

Purification and Labeling of Histones

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Expression and purification of Xenopus laevis core histone H2A, H2B, H3, and H4 and subsequent assembly of a histone octamer were performed following published protocols.^{39 (link)} H1G101C, a mutant of X. laevis linker histone H1° (termed H1 in this paper), was expressed and purified according to a previously published method.⁴⁰ After purification with a cation exchange column (Bio-Rex 70, 50–100 mesh, Bio-Rad Laboratories Inc., product no. 142-5832), H1G101C was labeled with the Cy5 maleimide monoreactive dye (GE Healthcare, product no. PA25031) according to the instructions recommended by the manufacturer. The labeling reaction mixture was purified with another cation exchange column (Bio-Rex 70, 100–200 mesh, Bio-Rad Laboratories Inc., product no. 142-5842) to remove any free dye. The labeling efficiency was close to 100% according to the absorbance values at 280 and 650 nm utilizing a correction factor of 0.05 for the dye at 280 nm. N-Terminally His₆-tagged yeast Nap1 (termed Nap1 in this paper) was overexpressed and purified according to previously published protocols.^{41 (link)} Briefly, yNap1 was purified with a Ni-NTA column (HisPur Ni-NTA, Thermo Scientific, product no. 88222) and a Mono-Q anion exchange column (Mono Q 4.6/100 PE, GE Healthcare, product code 17-5179-01).

Yue H., Fang H., Wei S., Hayes J.J, & Lee T.H. (2016). Single-Molecule Studies of the Linker Histone H1 Binding to DNA and the Nucleosome. Biochemistry, 55(14), 2069-2077.

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Extraction and Isolation of Tetrodontoxin Analogs

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TTX and its analogs were extracted from the visceral part of fish with 0.1% acetic acid by heating in a boiling water bath for 10 min, according to the method for TTX described in Standard Methods of Analysis in Food Safety, Japan [37 ]. TTX and its analogs, 4,9-anhydroTTX and 5,6,11-trideoxyTTX, were isolated by successive column chromatography on activated charcoal (for chromatography; Wako, Osaka, Japan), Bio-Gel P-2 (200–400 mesh; Bio-Rad, Hercules, CA, USA), and Bio-Rex 70 (200–400 mesh; Bio-Rad, Hercules, CA, USA) according to the method described by Nagashima et al., [14 (link)]. 4,9-anhydroTTX thus isolated was used to prepare the antigen. In addition, an aliquot of isolated TTX (ca. 10 µmol) was dissolved in 5 mL of 0.1% acetic acid and heated in boiling water for 30 min. 4-epiTTX and 4,9-anhydroTTX produced in the reaction mixture were isolated by Bio-Rex 70 column chromatography (200–400 mesh, 1.5 × 115 cm) and used as the materials for the examination of their cross-reactions with the polyclonal antibody against TTX, which was produced in the present study as described below. Moreover, 11-oxoTTX was prepared from the isolated TTX by treating with the FeSO₄ and H₂O₂ mixture, according to Wu et al. [40 (link)] (Figure S1).

Sato S., Takaishi S., Yasumoto K, & Watabe S. (2019). Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin. Toxins, 11(10), 551.

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Isolation and Extraction of Sugarcane Nuclei and Histones

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Sugarcane (Saccharum sp.) cultivar SP80-3280 was propagated from stem cuttings and grown in a 12 h light/12 h dark photoperiod at 26°C for six months. Nuclei were isolated from sugarcane according to Bowler et al. [31 (link)] with small modifications. Briefly, 40 g of material was blended in the presence of 300 ml of cold Nuclei grinding buffer (1 M hexylene glycol, 10 mM PIPES/KOH pH 7.0, 10 mM MgCl2, 10 mM 2-mercaptoethanol, 0.5 mM PMSF, 10 mM NaF, 20 mM sodium butyrate), Triton X-100 was then added to 0.5%. After 10 min incubation, the nuclei were pelleted by centrifugation for 10 min at 1000 g, resuspended in Nuclei wash buffer (0.5 M hexylene glycol, 10 mM PIPES/KOH pH 7.0, 10 mM MgCl2, 0.2% Triton X-100, 10 mM 2-mercaptoethanol, 0.5 mM PMSF, 10 mM NaF, 20 mM sodium butyrate) and pelleted again by centrifugation for 10 min at 4000 g. Bulk histones were then extracted from the nuclear pellet using the cation exchange resin Biorex-70 (Bio-rad) as described by Waterborg et al. [32 (link)].

Moraes I., Yuan Z.F., Liu S., Souza G.M., Garcia B.A, & Casas-Mollano J.A. (2015). Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane. PLoS ONE, 10(7), e0134586.

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Extraction and Purification of TTX and STX

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From the ovaries of T. rubripes, TTX with a purity greater than 60% was prepared, and from the xanthid crab Zosimus aeneus, STX with a purity greater than 80% was prepared by solvent partitioning, activated charcoal treatment, and Bio-Gel P-2 (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and Bio-Rex 70 (Bio-Rad) column chromatography according to the previously reported method [37 (link)], and used for the intrarectal TTX/STX administration experiment. Crystalline TTX (Wako Pure Chemical Industries Ltd., Tokyo, Japan) and an aqueous solution of STX purified from the xanthid crab Z. aeneus were used as external standards for the toxin quantification analyses described below.

Zhu H., Sakai T., Doi H., Yamaguchi K., Yamada A., Takatani T, & Arakawa O. (2023). Tetrodotoxin/Saxitoxin Accumulation Profile in the Euryhaline Marine Pufferfish Chelonodontops patoca. Toxins, 16(1), 18.

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Purification of E. coli RNA Polymerase

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All core RNAP subunits were expressed in E. coli BL21(DE3) cells transformed with pGEMABC (encoding rpoA, rpoB, and rpoC) and pACYCDuet-1_Ec_rpoZ (encoding rpoZ) (Murakami, 2013 (link)). Core RNAP was purified as follows. 16 g of cell paste was suspended in 50 ml of lysis buffer (50 mM Tris-HCl (pH 8 at 4 °C), 1 mM EDTA, 5 mM 2-mercaptoethanol, protease inhibitor mixture, and 2 mM PMSF), and cells were lysed using sonication. After a low-speed spin, RNAP in the soluble fraction was precipitated by adding 10% polyethyleneimine (Polymin P) solution (final concentration of 0.6%), and the pellet was recovered by low-speed centrifugation. RNAP was eluted from the pellet by suspension in TGED buffer (10 mM Tris-HCl (pH 8 at 4 °C), 10% glycerol, 0.1 mM EDTA, and 2 mM DTT) plus 1 M NaCl and then precipitated by ammonium sulfate (final 60% saturation). The pellet was suspended in TGED buffer and dialyzed against TGED buffer plus 50 mM NaCl. Core RNAP was purified by BioRex 70 (Bio-Rad), HiTrap Q (GE Healthcare), and Superdex 200 (GE Healthcare) column chromatography. E. coli σ⁷⁰ was expressed in BL21(DE3) cells transformed with pGEMD (Murakami, 2013 (link)). After cells were lysed by sonication, σ⁷⁰ was purified by HiTrap Q HP (GE Healthcare) and Superdex 200 column chromatography.

Molodtsov V., Sineva E., Zhang L., Huang X., Cashel M., Ades S.E, & Murakami K.S. (2018). Allosteric effector ppGpp potentiates the inhibition of transcript initiation by DksA. Molecular cell, 69(5), 828-839.e5.

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Stable Isotope Tracing in Marine Sciences

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¹⁵N-NaNO₃ (δ¹⁵N = 99.14%) was obtained from SRICI (Shanghai Research Institute of Chemical Industry CO., LTD., Shanghai, China). Bio-gel P-2 (400 mesh), Bio-Rex 70 (400 mesh) were obtained from BIO-RAD (Hercules, CA, USA). Certified reference materials for PSTs, including gonyautoxin 1/4 (GTX 1/4), gonyautoxin 2/3 (GTX 2/3), were purchased from the National Research Council, Institute for Marine Bioscience (Halifax, Canada). Analytical grade solvents were used for extraction purposes while LC grade solvents were used for HPLC-FLD applications.

Xie W., Li M., Song L., Zhang R., Hu X., Liang C, & Yang X. (2019). 15N Stable Isotope Labeling PSTs in Alexandrium minutum for Application of PSTs as Biomarker. Toxins, 11(4), 211.

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