Coulter act 10 hematology analyzer

Platelet rich plasma (PRP) was obtained by centrifuging whole blood at 0.3 G for 6 minutes at 4°C. Platelet concentrations of the PRP were quantified by a cell counter (Beckman Coulter, Brea, CA). PRP was aliquoted in 5 μL samples. Subsequently, 1 μL of CD41 (clone MWReg30, BD Biosciences, San Jose, CA) and CD62P antibodies (clone RB40.34, BD Biosciences, San Jose, CA) were added and allowed to incubate for 20 minutes. Following incubation, 500 μL of phosphate-buffered saline was added. All samples were transferred to flow tubbettes and read on the Attune Flow Cytometer (Life Technologies, Foster City, CA). Activated platelets were identified as CD41(+)/CD62P(+). Platelet counts for each sample were obtained at each timepoint utilizing a Coulter AcT 10 Hematology Analyzer (Beckman Coulter, Brea, California).

Whole blood collected via cardiac puncture from injured mice was anticoagulated with heparin. Coulter AcT 10 Hematology Analyzer (Beckman Coulter, Brea, California) was used to determine complete blood count, including white blood cell count, hemoglobin, hematocrit, and platelet count.

A complete blood count for each unit was obtained at each timepoint utilizing a Coulter AcT 10 Hematology Analyzer (Beckman Coulter, Brea, CA, USA). An arterial blood gas sample was analysed at each timepoint utilizing VetScan iSTAT (Abaxis, Union City, CA, USA). Prothrombin time evaluation was attempted, but did not generate a result using the iSTAT at any timepoint possibly because of an interaction of the storage solution with the iSTAT cartridge.