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Bessman tissue pulverizer

Manufactured by Thermo Fisher Scientific
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The Bessman Tissue Pulverizer is a laboratory equipment designed for efficient homogenization and pulverization of tissue samples. It utilizes a high-speed rotor to rapidly grind and disrupt tissue samples, preparing them for further analysis or processing.

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Lab products found in correlation

Luminex xmap technology, by DiaSorin (1 mentions) Mouse cytokine chemokine magnetic bead panel, by Merck Group (1 mentions) T per, by Thermo Fisher Scientific (1 mentions) Bca protein assay, by Merck Group (1 mentions) Magpix, by DiaSorin (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Serotonin, by Eagle Biosciences (1 mentions) Fastprep lysing matrix tubes, by MP Biomedicals (1 mentions) Complete mini protease inhibitor cocktail, by Roche (1 mentions)

3 protocols using bessman tissue pulverizer

1

Cytokine/Chemokine Profiling of Distal Colon

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Distal colon tissue isolated at euthanasia was snap frozen in liquid nitrogen and pulverized with a Bessman Tissue Pulverizer and placed in T-PER (Thermo Scientific, Rockfordm, IL, USA) with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Total protein was isolated by gravity centrifugation, 10,000× g for 5 min, of tissue fragments followed by collection of the buffer containing isolated protein, and storage at −80 °C until analysis. Protein concentrations were determined using a bicinchoninic acid (BCA) protein assay (Sigma-Aldrich, Milwaukee, WI, USA) according to the manufacturer’s protocol and normalized for all samples prior to loading on a Mouse Cytokine/Chemokine Magnetic Bead Panel (EMD Millipore, Billerica, MA, USA). The panel was analyzed with a Milliplex MAP Kit on a MagPix with Luminex xMAP technology (Luminex, Northbrook, IL, USA).
Royal J.M., Reeves M.A, & Matoba N. (2019). Repeated Oral Administration of a KDEL-Tagged Recombinant Cholera Toxin B Subunit Effectively Mitigates DSS Colitis despite a Robust Immunogenic Response. Toxins, 11(12), 678.
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2

Cartilage Protein Expression Analysis

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Cartilage samples from human and bovine knee joints to be used for immunoblotting were flash-frozen in liquid nitrogen, pulverized with a Bessman tissue pulverizer (Fischer Scientific), chilled in liquid Nitrogen, and put into Lysis Buffer optimized for cartilage protein extraction as previously described [18 (link)]. Proteins were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and probed for the presence of Runx1 and Gapdh. We analyzed 58 lateral and 52 human cartilage samples from n=5 patients which were compiled for statistical analysis. For bovine experiments, 12 cartilage discs were harvested from the same compression chamber and pooled together for protein isolation. Densitometry analysis (HP Scanjet 7400c using transilluminator XPA attachment) of immunoblots were performed for the Runx1 and corresponding GAPDH for normalization of each individual sample.
LeBlanc K.T., Walcott M.E., Gaur T., O’Connell S.L., Basil K., Tadiri C.P., Mason-Savas A., Silva J.A., van Wijnen A.J., Stein J.L., Stein G.S., Ayers D.C., Lian J.B, & Fanning P.J. (2015). Runx1 Activities in Superficial Zone Chondrocytes, Osteoarthritic Chondrocyte Clones and Response to Mechanical Loading. Journal of cellular physiology, 230(2), 440-448.
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3

Cecal Tissue Serotonin and Glutamate Analysis

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Cecal tissue samples (100 mg) were pulverized using a Bessman Tissue pulverizer (ThermoFisher Scientific) followed by homogenization in cold lysis buffer (100-mg tissue/mL; 20-mM Tris, 0.25M sucrose, 2.0-mM EDTA, 10-mM EGTA, 1.0% Triton X-100) containing Complete Mini protease inhibitor cocktail (one tablet/10 mL; Roche Diagnostics, Indianapolis, IN) using FastPrep® Lysing Matrix tubes (MP Biomedicals). Homogenates were clarified by centrifugation at 21 000 × g for 40 minutes at 4°C. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to determined levels of serotonin (Eagle Biosciences, Nashua, NH) and glutamate (Abnova, Taiwan) in the cecal tissue homogenate according to the manufacturer’s protocol.
Vetreno R.P., Massey V, & Crews F.T. (2019). Long-lasting microbial dysbiosis and altered enteric neurotransmitters in adult rats following adolescent binge ethanol exposure. Addiction biology, 26(1), e12869.
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