Freshly isolated hUC-MSC-EVs in 150 μl of 1xPBS suspension were loaded onto carbon-coated copper grids (Ted Pella, USA) and incubated for 10 min. The grid was blotted with filter paper and stained with 2% uranyl acetate (Ted Pella, USA) for 1 min. Excessive uranyl acetate was removed, and the grid was let dry for 15 min before viewing using Energy Filter TEM Libra-120 (Carl Zeiss AG, Germany).
Uranyl acetate
Manufactured by Zeiss
Sourced in Germany
Uranyl acetate is a chemical compound commonly used in the field of electron microscopy as a staining agent. It is a yellow crystalline solid that is soluble in water and various organic solvents. Uranyl acetate is primarily used to enhance the contrast of biological samples during the preparation and imaging process in electron microscopy.
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Lab products found in correlation
5 protocols using uranyl acetate
1
Transmission Electron Microscopy of hUC-MSC-EVs
2
Ultrastructural Evaluation of Ovarian Tissue
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All chemicals were obtained from TAAB Laboratories Ltd. (Berkshire, UK). Vitrified and non-vitrified ovaries were randomly collected (n=3 from each groups) after equilibration in medium for 30 min and fixed in 2.5% glutaraldehyde in PBS (pH=7.4) for 2 hr, and post fixed with 1% osmium tetroxide in the same buffer for 2 hr. Following dehydration in an ascending series of ethanol, specimens were placed in propylene oxide and embedded in Epon 812. The ovarian tissue was cut into 0.5 μm sections (semi-thin sections), stained with toluidine blue and observed in light microscopy. Ultrathin sections (60–80 nm) were contrasted with uranyl acetate and lead citrate for evaluation by electron microscopy (Zeiss-Gottingen-Germany).
The granulosa cells and oocyte were studied to find the integrity of the cytoplasmic membrane, the mitochondrial cristae, nuclear membrane, density of cytoplasm, cytoplasmic organelles, the numbers and size of vesicles in the cytoplasm and attachment of the cytoplasm to granulosa cells.
The granulosa cells and oocyte were studied to find the integrity of the cytoplasmic membrane, the mitochondrial cristae, nuclear membrane, density of cytoplasm, cytoplasmic organelles, the numbers and size of vesicles in the cytoplasm and attachment of the cytoplasm to granulosa cells.
3
Ultrastructural Analysis of hUC-MSC-EV
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Freshly isolated hUC-MSC-EV in 150µl of 1xPBS suspension were loaded onto carbon-coated copper grids (Ted Pella, USA) and incubated for 10 minutes. The grid was blotted with lter paper and stained with 2%
uranyl acetate (Ted Pella, USA) for 1 minute. Excessive uranyl acetate was removed and the grid was let dry for 15 min before viewing using Energy Filter TEM Libra-120 (Carl Zeiss AG, Germany).
uranyl acetate (Ted Pella, USA) for 1 minute. Excessive uranyl acetate was removed and the grid was let dry for 15 min before viewing using Energy Filter TEM Libra-120 (Carl Zeiss AG, Germany).
4
Transmission Electron Microscopy of hUC-MSC-EVs
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Freshly isolated hUC-MSC-EVs in 150µl of 1xPBS suspension were loaded onto carbon-coated copper grids (Ted Pella, USA) and incubated for 10 minutes. The grid was blotted with lter paper and stained with 2% uranyl acetate (Ted Pella, USA) for 1 minute. Excessive uranyl acetate was removed, and the grid was let dry for 15 min before viewing using Energy Filter TEM Libra-120 (Carl Zeiss AG, Germany).
5
Transmission Electron Microscopy of hUC-MSC-EVs
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Freshly isolated hUC-MSC-EVs in 150µl of 1xPBS suspension were loaded onto carbon-coated copper grids (Ted Pella, USA) and incubated for 10 minutes. The grid was blotted with lter paper and stained with 2% uranyl acetate (Ted Pella, USA) for 1 minute. Excessive uranyl acetate was removed, and the grid was let dry for 15 min before viewing using Energy Filter TEM Libra-120 (Carl Zeiss AG, Germany).
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