Samples of venous blood were drawn from antecubital veins in supine position on hospital admission, before coronary angiography and percutaneous coronary intervention (PCI) were performed. Samples were centrifuged for serum isolation. Standard biochemical and hematological blood test were performed using an automated blood cell counter with Sysmex XN-1000, CK-MB was measured using the immunological UV assay method with Cobas c501 (Roche Diagnostics, Switzerland), Troponin I was measured using the enzyme-linked fluorescent assay (ELFA) method with MiniVIDAS (bioMerieux, France). Aliquote of serum was stored at −80’ C until thawed for determination of sST2. sST2 levels were measured using Quantikine Human ST2/IL-33 R Immunoassay (R&D Systems, Minneapolis, MN, USA). The testing was performed by personnel blinded to patient characteristics and clinical outcome.
Quantikine human st2 il 33 r immunoassay
Manufactured by R&D Systems
Sourced in United States
The Quantikine Human ST2/IL-33 R Immunoassay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure human ST2 or IL-33 receptor levels in cell culture supernates, serum, and plasma.
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Lab products found in correlation
2 protocols using quantikine human st2 il 33 r immunoassay
1
Biomarker Profiling in Coronary Artery Disease
2
Plasma sST2 Levels in T2DM Patients
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Non-diabetic controls were matched with T2DM patients on age (+/-5 years) and sex. They originated either from a study of healthy volunteers or from patients from the Poitiers Biobank, hospitalized for non-malignant adrenal incidentaloma or pituitary disease, without conditions such as infection, inflammatory disease or cancer. Plasma sST2 levels were measured using the Quantikine Human ST2/IL33 R Immunoassay (R&D Systems), according to the manufacturer's instructions.
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