In order to assess the degree of atrophy of the muscles innervated by the operated left sciatic nerve, the weight (w) and volume (v) of the whole lower leg was measured at 14 weeks after surgery. Lower legs were harvested after the intracardiac perfusion (see details below). The lower legs [which contain several muscles exclusively innervated by the sciatic nerve trunk (Greene, 1968 )] were exposed and then disjointed from the knee and ankle. For the w assessment, the dissected legs were removed from the fixative, dried in absorbent paper and weighed in a digital weighing machine (Sartorius BP 121S, precision: 0.1 mg, Sigma–Aldrich). For the v assessment, dissected legs were immersed in test tube (50 ml) containing 30 ml of PBS and the increase of this volume represented the lower legs volume. In this study, the percentage of w and v loss was calculated between the operated and the contralateral-side leg from each animal, including healthy animals (CTR-Native), and these values were used for statistical comparisons.
Bp121s
Manufactured by Sartorius
Sourced in Germany
The BP121S is a digital balance from Sartorius. It has a weighing capacity of 120 g and a readability of 0.1 mg. The balance features a stainless steel weighing pan and a backlit LCD display.
Automatically generated - may contain errors
Lab products found in correlation
Low temperature centrifuge, by Thermo Fisher Scientific (3 mentions)
Uv imaging system, by Analytik Jena (2 mentions)
Trizol kit and reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Ultraviolet imaging system, by Analytik Jena (2 mentions)
Pipettor, by Eppendorf (2 mentions)
Reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Trizol kit, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Mdivi 1, by Selleck Chemicals (1 mentions)
Frozen slicer, by Leica (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
80 c refrigerator, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase labeled anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions)
Cell culture flask, by Corning (1 mentions)
Rabbit anti gsk 3β, by Cell Signaling Technology (1 mentions)
Mmp 9 elisa kit, by R&D Systems (1 mentions)
Rabbit anti pgsk3β ser9, by Cell Signaling Technology (1 mentions)
Rabbit anti dkk1, by Cell Signaling Technology (1 mentions)
Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
9 protocols using bp121s
1
Sciatic Nerve Atrophy Evaluation
2
Muscle Atrophy Quantification Protocol
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To assess the degree of muscle atrophy, the whole lower leg was separated from the surrounding skin and dissected from the knee and ankle then fixed immediately in 10% buffered formalin for at least 1 week. The legs were weighted using a digital balance (Sartorius BP121 S, precision level = 0.1 mg, Sigma-Aldrich). The volume was measured following Archimede’s principle and fluid displacement rules, where a cylinder was filled with 30 mL PBS and the leg was immersed totally in the cylinder, the volume of the immersed leg is equal to the volume of liquid displacement. For each rat, the operated leg and contralateral leg were measured, and the percentage of loss was calculated as [100 – (operated leg/contralateral leg × 100)].
3
Thermal Analysis of HC Samples
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The TGA study was carried out on a NETZSCH STA 449 F1 Jupiter simultaneous thermal analysis instrument (Selb, Germany). The HC samples were analyzed in argon at a heating rate of 10 °C min−1, temperatures from 30 to 900 °C, and protective and blowout gas flow rates of 20 and 50 mL min−1, respectively. The Al2O3 cylindrical crucible with a perforated cover was used and a reference was an empty corundum crucible with a cover. The instrument was calibrated according to the specification using reference substances supplied with the instrument. The sample weight for the analysis was determined on a Sartorius BP121S analytical lab scale digital balance. The measurement data were processed using the NETZSCH. Proteus Thermal Analysis.5.1.0 software supplied with the instrument.
4
Solid Dosage Form Evaluation
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The prepared tablets were evaluated for hardness value (Sotax HT1), diameter (digital caliper, Mituyoto), friability percent (Erweka), average weight (Sartorius BP 121S) and drug content uniformity according to US Pharmacopeia XXXI (2008) , as shown in Table 2 .
5
Mdivi-1 Inhibits Inflammation in Rats
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Mdivi-1 (Selleck, Houston, TX, USA); TRIzol kit, rat anti-Drp1 antibody, enzyme-linked immunosorbent assay (ELISA) kit for interleukin-6 (IL-6) and ELISA kit for tumor necrosis factor-α (TNF-α) (all from BD Biosciences, Franklin Lakes, NJ, USA); secondary antibody against rat (Beijing Zhongshan Golden Bridge Biotech Co., Ltd., Beijing, China); rabbit anti-IL-6 (1:500; cat. no. 12153), rabbit anti-TNF-α (1:500; cat. no. 8184), rabbit anti-MCP-1 (1:500; cat. no. 39091), horseradish peroxidase-labeled secondary antibody against rabbit (1:1,000; cat. no. 7074) (all from Cell Signaling Technology Co., Ltd.); electrochemiluminescence (ECL) solution and color development powder (both from Invitrogen, Carlsbad, CA, USA); pipette (Eppendorf, Hamburg, Germany); electronic scale (BP121S; Sartorious, Goettingen, Germany); −80°C refrigerator and low-temperature centrifuge (Thermo Fisher Scientific, Dreieich, Germany); other instruments and reagents are indicated in the corresponding part in this study.
6
Autophagy Pathway Evaluation in Kidney Injury
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Creatinine (Cr) assay kit and blood urea nitrogen (BUN) assay kit (R&D Systems, Inc., Minneapolis, MN, USA), 3-MA (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); TRIzol kit and reverse transcription kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA); semi-quantitative PCR kit (Vazyme Biotech Co., Ltd., Nanjing, China); horseradish peroxidase-labeled anti-rabbit secondary polyclonal antibody (#7074), rabbit anti-mouse Beclin1, rabbit anti-p62, rabbit anti-mouse LC3-II, rabbit anti-LC3-I and rabbit anti-mouse GAPDH monoclonal antibodies (cat. nos. #3495, #39749, #3868, #4599, #2118; Cell Signaling Technology, Inc., Danvers, MA, USA); ECI luminous fluid and contrasting power (Invitrogen; Thermo Fisher Scientific, Inc.); pipettes (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems; Thermo Fisher Scientific, Inc., Waltham, MA, USA); UV imaging system (Biometra, Göttingen, Germany); electronic balance (BP121S, Sartorious, Göttingen, Germany); low temperature centrifuge (Thermo Fisher Scientific, Inc.); frozen slicer (Leica Microsystems GmbH, Wetzlar, Germany).
7
Desflurane Effect on Neuroinflammation
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Desflurane (Shenzhen RWD Life Science Co., Ltd., Shenzhen China); dimethylsulfoxide (DMSO) (Sigma, St. Louis, MO, USA); TRIzol kit; reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA); rabbit anti-rat p38, IL-1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) polyclonalantibodies; anti-rabbit horseradish peroxidase-labeled rabbit secondary polyclonal antibody (cat. nos. 8690, 12703, 2118 and 7074; both from Cell Signaling Technology, Beverly, MA, USA); electrochemiluminescence (ECL) liquid; developing powder (both from Invitrogen); skim milk powder (Guangzhou Sijia Biotechnology Co., Ltd., Guangzhou, China); polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA); pipettor (Eppendorf, Hamburg, Germany); polymerase chain reaction (PCR) instrument (ABI, Foster City, CA, USA); ultraviolet imaging system (Biometra, Goettingen, Germany); electronic balance (BP121S; Sartorious, Göttingen, Germany); −80°C refrigerator (Thermo Fisher Scientific, Darmstadt Germany); instruments of novel object recognition test and Morris water maze (Guangzhou Jiebeisi Animal Instrument Co., Ltd., Guangzhou, China); low-temperature centrifuge (Thermo Fisher Scientific); other related equipment and reagents are described in relevant parts.
8
Oxcarbazepine Pharmacokinetics and Molecular Mechanisms
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Oxcarbazepine tablets (Novartis Farma S.p.A, Switzerland); dimethylsulfoxide (DMSO) (Sigma); TRIzol kit (Invitrogen); reverse transcription kit (Invitrogen, USA); ELISA kit (R&D system, USA); electrochemiluminescence (ECL) solution (Invitrogen, USA); rabbit anti-Ppg, rabbit anti-MRP1 and rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology); horseradish peroxidase-labeled anti-rabbit secondary antibody (Cell Signaling Technology); polymerase chain reaction (PCR) instrument (Applied Biosystems, USA); ultraviolet imaging system (Biometra, Germany); electronic balance (BP121S, Sartorious, Germany); other relevant equipment and reagents were illustrated in relevant parts.
9
Osteosarcoma Cell Line 143B Cytotoxicity
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Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. Any other equipment and reagents were previously described in the relevant section.
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