G 5555

Manufactured by MedChemExpress

Sourced in United States

G-5555 is a laboratory equipment product designed for conducting various experiments and analyses. It serves as a versatile tool in research and development environments. The core function of G-5555 is to provide a controlled and standardized platform for scientific investigations, without any specific interpretation or extrapolation on its intended use.

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Lab products found in correlation

3 protocols using g 5555

Evaluating PAK1 Kinase Inhibitors

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CP734 was purchased from Target Molecule Corp. (Boston, MA, USA); FRAX597 and G-5555 were from MedChemExpress (Shanghai, China); Z′-LYTE^TM biochemical assay kit from Invitrogen (Carlsbad, CA, USA); human recombinant protein kinase PAK1 from BPS Bioscience Inc. (San Diego, CA, United States); Primary antibodies against p-PAK1 (Lot. #2601), PAK1 (Lot. #2602), p-c-RAF (Lot. #9427), c-RAF (Lot. #9422), p-AKT: (Lot. #4060), AKT (Lot. #9272), cyclin D1 (Lot. #2922), c-MYC (Lot. #9402), p-P53 (Lot. #2521), P53 (Lot. #9282), P21 (Lot. #2946), PUMA (Lot. #4976), cyclin B1 (Lot. #4138), and β-actin (Lot. #3700) were purchased from Cell Signaling Technology (Danvers, MA, USA). The secondary antibodies were also from Cell Signaling Technology. All reagents for cell culture were purchased from Gibco (Thermofisher, Rockville, MA, USA).

Wang J., Zhu Y., Chen J., Yang Y., Zhu L., Zhao J., Yang Y., Cai X., Hu C., Rosell R., Sun X, & Cao P. (2019). Identification of a novel PAK1 inhibitor to treat pancreatic cancer. Acta Pharmaceutica Sinica. B, 10(4), 603-614.

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Inhibition of PAK, BRAF, and AKT

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The following drugs were used: FRAX1036, group I PAK inhibitor (generous gift from Dr. Jonathan Chernoff, Fox Chase Cancer Center, PA); G-5555, group I PAK inhibitor (MedChemExpress, Monmouth Junction, NJ, HY-19635); PLX4032, BRAF^V600E inhibitor (Selleckchem, S1267), MK2206, AKT inhibitor (MedChemExpress, HY-10358). For in vitro assays, drugs were reconstituted in DMSO.

Knippler C.M., Saji M., Rajan N., Porter K., La Perle K.M, & Ringel M.D. (2019). MAPK- and AKT-activated thyroid cancers are sensitive to group I PAK inhibition. Endocrine-related cancer, 26(8), 699-712.

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Evaluating PAK1 Inhibitor Efficacy

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The PAK1 small‐molecule inhibitor FRAX‐597 (S7271) was purchased from Selleck Chemicals (Houston, TX, USA), and G‐5555 (HY‐19635) was purchased from MedChemExpress, LLC (Monmouth Junction, NJ, USA). DMSO was used as a vehicle control. For drug efficacy evaluation, 1 µm of FRAX‐597 or G‐5555 was incubated with the cell lines for 3 h. For migration assays, 1 µm of each drug was incubated with the starved cells before the assay. Migrated cells were stained and counted after 3 h of incubation.

Chen X., Cates J.M., Du Y.C., Jain A., Jung S.Y., Li X.N., Hicks J.M, & Man T. (2020). Mislocalized cytoplasmic p27 activates PAK1‐mediated metastasis and is a prognostic factor in osteosarcoma. Molecular Oncology, 14(4), 846-864.

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