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Anti msi2 ab76148

Manufactured by Abcam
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Anti-MSI2 (#ab76148) is a rabbit polyclonal antibody that recognizes the MSI2 protein. MSI2 is an RNA-binding protein involved in the regulation of stem cell maintenance and differentiation. The antibody can be used to detect the expression of MSI2 in various cell and tissue types.

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Lab products found in correlation

Osimertinib, by Selleck Chemicals (1 mentions) Doxycycline, by Merck Group (1 mentions) Phospho erk t202 y204, by Cell Signaling Technology (1 mentions) Normal rabbit igg, by Cell Signaling Technology (1 mentions) Phospho akt t308, by Cell Signaling Technology (1 mentions) Phospho p70s6k t389, by Cell Signaling Technology (1 mentions) Superase in rnase inhibitor, by Thermo Fisher Scientific (1 mentions) Her2 erbb2, by Cell Signaling Technology (1 mentions) Her3 erbb3, by Cell Signaling Technology (1 mentions) Smad3, by Cell Signaling Technology (1 mentions) Total erk, by Cell Signaling Technology (1 mentions) Total p70s6k, by Cell Signaling Technology (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) Afatinib, by LC Laboratories (1 mentions) Erlotinib, by LC Laboratories (1 mentions) Anti mouse hrp linked, by Cell Signaling Technology (1 mentions) Anti vegfr2, by Cell Signaling Technology (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions) Anti phakt t308, by Cell Signaling Technology (1 mentions) Anti phakt s473, by Cell Signaling Technology (1 mentions)

2 protocols using anti msi2 ab76148

1

Antibody and Protein Assay Protocol

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Anti-MSI2 (#ab76148), anti-MSI1 (#ab21628), and anti-β-actin HRP conjugated (#ab49900) antibodies and recombinant MSI2 protein (#ab167853) were obtained from Abcam, (Cambridge, UK). Anti-EGF receptor (#4267), phospho-EGFR (Y1068) (mAb #2234), HER3/ErbB3 (#12708), HER2/ErbB2 (#4290), Smad3 (#9523), phospho-AKT (T308) (#13038), total AKT (#2920), phospho-ERK (T202/Y204) (#4370), total ERK (#4696), phospho-p70S6K (T389) (#9234), total p70S6K (#2708), and normal Rabbit IgG (#2729) were obtained from Cell Signaling, (Danvers, MA). Erlotinib and afatinib were obtained from LC Laboratories (Woburn, MA), doxycycline from Sigma-Aldrich (D9891, Darmstadt, Germany). SUPERase-In RNAse inhibitor was obtained from Thermo Fisher Scientific, (AM2694 Waltham, MA). Osimertinib was obtained from Selleckchem (#S7297, Houston, TX)
Makhov P., Bychkov I., Faezov B., Deneka A., Kudinov A., Nicolas E., Brebion R., Avril E., Cai K.Q., Kharin L.V., Voloshin M., Frantsiyants E., Karnaukhov N., Kit O.I., Topchu I., Fazliyeva R., Nikonova A.S., Serebriiskii I.G., Borghaei H., Edelman M., Dulaimi E., Golemis E.A, & Boumber Y. (2021). Musashi-2 (MSI2) regulates epidermal growth factor receptor (EGFR) expression and response to EGFR inhibitors in EGFR-mutated non-small cell lung cancer (NSCLC). Oncogenesis, 10(3), 29.
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2

Molecular Signaling Pathway Analysis

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Anti-MSI2 (#ab76148) and anti-VEGF-A (#ab46154) were obtained from Abcam (Cambridge, UK). Anti-VEGFR2 (#2479), anti-phVEGFR2 (#3817), anti-β-actin (#3700), anti-phAKT (T308) (#13038), anti-phAKT (S473) (#4060), anti-AKT total (#2920), anti-phGSKαβ (#9331), anti-GSKαβ total (#5676), anti-PTEN (#14642), anti-phERK (#4370), anti-ERK total (4695), anti-ph4EBP(#2855), anti-phP70SK (#9206), anti-P70S6K total (#2708), anti-rabbit HRP-linked (#7074), and anti-mouse HRP-linked (#7076) were obtained from Cell Signaling (Danvers, MA, USA). Doxycycline (#HY-N0565), hygromycin (HY-B0490), and puromycin (HY-B1743A) were obtained from MedChemExpress (Monmouth Junction, NJ, USA).
Bychkov I., Topchu I., Makhov P., Kudinov A., Patel J.D, & Boumber Y. (2023). Regulation of VEGFR2 and AKT Signaling by Musashi-2 in Lung Cancer. Cancers, 15(9), 2529.
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