FACS data acquisition were performed in FACSDiva v6.1.3 (BD) and analyzed in FlowJo v7.6.3 (FLOWJO LLC). Quantitative PCR data acquisition were performed in QuantStudio Real-Time PCR Software v1.3 (Applied Biosystems). Force measurements were acquired using LabChart v6.1.1 (ADInstruments). Immunostaining data were acquired using ZEN v2.3 pro (Zeiss). Fiber counting and measurements were performed with ImageJ v2.0.0-rc-65/1.52a (NIH).
Labchart v6
Manufactured by ADInstruments
Sourced in United Kingdom, Australia
LabChart V6 is a data acquisition and analysis software developed by ADInstruments. It is designed to record, visualize, and analyze physiological and biological data from a variety of instruments and sensors.
Automatically generated - may contain errors
5 protocols using labchart v6
1
Multimodal Data Acquisition Protocol
2
Integrated Cardiopulmonary and Cardiac MRI
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Firstly, cardiopulmonary exercise testing with continuous monitoring of expiratory gases was performed on an upright cycle ergometer (ER900 and Oxycon Alpha, Jaeger, Germany). Through breath‐by‐breath analysis minute ventilation, oxygen consumption and carbon dioxide production were assessed. Additional measures included peak heart rate, peak power, and the ventilatory equivalent for carbon dioxide. Secondly, within 24 h, all subjects underwent exercise CMR imaging with simultaneous invasive pressure measurements through a 7‐Fr MRI‐compatible pulmonary artery catheter (Edwards Lifesciences, CA, USA) and a 20‐G radial arterial catheter. Pressures were recorded through MRI‐compatible transducers connected to a PowerLab recording system (ADInstruments, Oxford, UK) and analysed offline using LabChart V6.1.1 (ADInstruments, Oxford, UK). Images were acquired during free breathing at rest, and during exercise at 25%, 50% of peak power achieved during CPET. Workloads were imposed nearly instantaneously and were maintained for ≈3 min at each stage, 30 s to achieve a physiological steady state, and then 2–3 min for image acquisition and will hereinafter be referred to as rest, low, and moderate intensity.
3
Assessing Forearm Vascular Responses
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Strain-gauge plethysmography was used to measure forearm blood flow as described previously (30) (link). For each study, the brachial artery of the nondominant arm was cannulated with a 27-gauge needle (Cooper’s Needle Works, Birmingham, United Kingdom) under local anesthesia (3 ml of 1% lignocaine). Drugs or normal saline (sodium chloride 0.9% wt/vol) were infused continuously at 1 ml/min. During recording periods, the hands were excluded from the circulation by inflation of wrist cuffs to 200 mm Hg. Responses to both acetylcholine (Ach) (25, 50, and 100 nmol/min) and glyceryltrinitrate (GTN) (4, 8, and 16 nmol/min) were assessed before and after combined rIPC and IR. All recordings and analysis were made using LabChart v.6 (AD Instruments, Chalgrove, United Kingdom).
4
Preterm Neonatal Sleep Assessment
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Preterm neonates underwent a 2-h sleep study in the neonatal intensive care unit on postnatal day 1. Continuous electrocardiograms (ECG) as part of routine clinical care were collected and digitalized for analysis (PowerLab 16SP, ADInstruments, New South Wales, Australia; LabChart V6, ADInstruments, Sydney, New South Wales, Australia). A sampling rate at or above 512 Hz was used. Neonates were studied in the supine position and sleep state was scored at cot side as AS or QS based on behavioral criteria (44) . Owing to the logistic reasons, no data were acquired in term AGA neonates on postnatal day 1.
5
Infant Sleep Polysomnography and Vital Monitoring
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All groups underwent daytime polysomnography in our sleep laboratory at 1 and 6 months post-term age. ECG data were recorded with a sampling rate of 512 Hz. In addition to ECG, electrophysiological signals required for sleep state scoring were recorded and included electroencephalogram, electro-oculogram, submental electromyogram, and abdominal and thoracic respiratory effort (zRIP Durabelt Pediatric, Pro-Tech, Mukilteo, WA). Arterial oxygen saturation was recorded via pulse oximetry (Masimo Australia Pty Ltd, Frenchs Forest, NSW, Australia). In addition to standard polysomnographic recordings, blood pressure was measured noninvasively and continuously using a photoplethysmographic cuff placed around the infant's wrist (Finometer, FMS, Finapres Medical Systems, the Netherlands). To avoid venous pooling in the infant's hand, blood pressure was recorded in 2 min epochs, followed by a 2 min rest period (45) . All infants were studied in the supine position. PSG data were recorded using a commercially available PSG system (E-Series; Compumedics, Melbourne, Victoria, Australia). Data were transferred via EDF format to LabChart V6 (ADInstruments, Sydney, New South Wales, Australia) for analysis. Sleep state was scored off-line using standard infant criteria (46) .
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