Fxia chromogenic substrate s 2366

Human CatG was purchased from Enzo life sciences (Farmingdale, NY). Human plasma clotting enzymes and digestive enzymes were purchased from Haematologic Technologies (Essex Junction, VT). Laminin, fibronectin and the chromogenic substrate for CatG (S-7388) were purchased from Sigma Aldrich (St. Louis, MO). The chromogenic substrates for thrombin, FIIa, FVIIa, FXa, FIXa and FXIIa were purchased from Biomedica Diagnostics (Windsor, NS Canada). FXIa chromogenic substrate (S-2366) and trypsin chromogenic substrate (S-2222) were purchased from Diapharma (West Chester, OH). Pre-cast SDS-PAGE gels were from BioRad (Hercules, CA). Coomassie brilliant blue for gel electrophoresis was from Fisher Scientific (Waltham, MA). Stock solutions of CatG were prepared in 20 mM Tris-HCl buffer, pH 7.4 containing 0.02% Tween80, 0.1% PEG8000, 2.5 mM CaCl₂ and 100 mM NaCl. All experiments were repeated at least two times.

Pentamidine isethionate was purchased from Millipore-Sigma (St. Louis, MO, USA). Reagents for clotting assays including thromboplastin-D, APTT reagent, and thrombin time reagent were all from Fisher Scientific (Pittsburgh, PA, USA). Chemicals used to prepare enzyme assay buffers were from Millipore-Sigma or Fisher Scientific. N,N–dimethylcasein, dansylcadaverine, and dithiothreitol were also from Millipore-Sigma. All types of plasmas were purchased from George King Bio-Medical, Inc. (Overland Park, KS, USA). Coagulation enzymes including thrombin, factor Xa (FXa), factor XIa (FXIa), and factor XIIIa (FXIIIa) were from Haematologic Technologies, Inc. (Essex Junction, VT, USA). Digestive enzymes including trypsin and chymotrypsin were from Millipore-Sigma. Neutrophil elastase was from Elastin Products Company (Owensville, MO, USA). Chromogenic substrates: Spectrozyme TH, Spectrozyme FXa, and Spectrozyme PL, were obtained from Biomedica-Diagnostics (Windsor, NS, Canada). FXIa chromogenic substrate (S-2366) and trypsin substrate (S-2222) were obtained from Diapharma (West Chester, OH, USA). Chromogenic substrate for chymotrypsin (N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide) and that for neutrophil elastase (S-1384) were from Millipore-Sigma.