Mts assay kit celltiter 96 aqueous one solution reagent

Sorafenib (Selleck Chemicals, Houston, TX, USA), auranofin (MCE, Houston, TX, USA), and Gefitinib (Selleck Chemicals, Houston, TX, USA) were dissolved in DMSO at a stock concentration of 10 mM, Erlotinib (Selleck Chemicals, Houston, TX, USA) was dissolved in DMSO at a stock concentration of 5 mM and stored in aliquots at −20 °C. MTS assay Kit (CellTiter 96 Aqueous One Solution reagent) was purchased from Promega Corporation (Madison, WI, USA). Annexin V-FITC/PI apoptosis Detection Kit was purchased from Keygen Company (Nanjing, China). Mito-SOX were purchased from Invitrogen (Carlsbad, CA, USA). Anti-TXNRD2 (ab16841), anti-Trx2 (ab185544), anti-MCL-1 (ab32087), and anti-TXNRD3 (ab134034) antibodies were purchased from Abcam (Cambridge, MA, USA); Anti-HSP60 (#4870S), anti-TXNRD1 (#15140s), anti-α-tubulin (#2144), anti-β-actin (#4970), anti-Bcl-2 (#3498s), and anti-Bcl-xL (#2762s) antibodies were from Cell Signaling Technologies (Danvers, MA, USA).