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Dapi uv nuclear stain

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DAPI (UV) is a fluorescent dye that binds to the minor groove of DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize the cell nucleus.

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Lab products found in correlation

Acta2, by Abcam (2 mentions) Triton x 100, by Avantor (2 mentions) Robo4, by Abcam (2 mentions) Robo4, by Santa Cruz Biotechnology (2 mentions) Draq5 far red nuclear stain, by Enzo Life Sciences (2 mentions) Pecam 1, by Santa Cruz Biotechnology (2 mentions)

2 protocols using dapi uv nuclear stain

1

Immunofluorescent Staining of Endothelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were fixed in 4% paraformaldehyde overnight at 4°C.
Samples were then washed for 15 minutes on a rocker 3 times with PBS,
permeabilized with 0.2% Triton-X 100 (VWR International, Radnor, PA) for 10
minutes, and washed another 3 times with PBS. Samples were incubated overnight
at 4°C in a 1% BSA (Rockland Immunochemicals) blocking solution followed
by another 4°C overnight incubation with ROBO4 (Abcam, ab103674), ROBO4
(Santa Cruz, sc-46497), CD31 (Biocare Medical, CM303A), PECAM1 (Santa Cruz,
sc-1506), CDH5 (Santa Cruz, sc-28644), TJP1 (Santa Cruz, sc-8146), ACTA2 (Abcam,
ab7817), or ALB (Santa Cruz, sc-46293). After 3 washes for 15 minutes with PBS,
samples were exposed to Alexa Fluor 488 or 568 conjugated (Invitrogen),
species-specific secondary antibodies at 1:100 in 1% BSA for 2 hours at room
temperature. Three more washes with PBS for 15 minutes were followed by
incubation with either DRAQ5 (far-red) nuclear stain or DAPI (UV) nuclear stain
(Enzo Life Sciences) at 1:1000.
Gould R.A., Aziz H., Woods C.E., Seman-Senderos M.A., Sparks E., Preuss C., Wünnemann F., Bedja D., Moats C.R., McClymont S.A., Rose R., Sobeira N., Ling H., MacCarrick G., Anand Kumar A., Luyckx I., Cannaerts E., Verstraeten A., Björk H.M., Lehsau A.C., Jaskula-Ranga V., Lauridsen H., Shah A.A., Bennett C.L., Ellinor P.T., Lin H., Isselbacher E.M., Cardenas C.L., Butcher J.T., Hughes G.C., Lindsay M.E., Mertens L., Franco-Cereceda A., Verhagen J.M., Wessels M., Mohamed S.A., Per E., Mital S., Laer L.V., Loeys B.L., Andelfinger G., McCallion A.S, & Dietz H.C. (2018). ROBO4 Variants Predispose Individuals to Bicuspid Aortic Valve and Thoracic Aortic Aneurysm. Nature genetics, 51(1), 42-50.
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2

Immunofluorescent Staining of Endothelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were fixed in 4% paraformaldehyde overnight at 4°C.
Samples were then washed for 15 minutes on a rocker 3 times with PBS,
permeabilized with 0.2% Triton-X 100 (VWR International, Radnor, PA) for 10
minutes, and washed another 3 times with PBS. Samples were incubated overnight
at 4°C in a 1% BSA (Rockland Immunochemicals) blocking solution followed
by another 4°C overnight incubation with ROBO4 (Abcam, ab103674), ROBO4
(Santa Cruz, sc-46497), CD31 (Biocare Medical, CM303A), PECAM1 (Santa Cruz,
sc-1506), CDH5 (Santa Cruz, sc-28644), TJP1 (Santa Cruz, sc-8146), ACTA2 (Abcam,
ab7817), or ALB (Santa Cruz, sc-46293). After 3 washes for 15 minutes with PBS,
samples were exposed to Alexa Fluor 488 or 568 conjugated (Invitrogen),
species-specific secondary antibodies at 1:100 in 1% BSA for 2 hours at room
temperature. Three more washes with PBS for 15 minutes were followed by
incubation with either DRAQ5 (far-red) nuclear stain or DAPI (UV) nuclear stain
(Enzo Life Sciences) at 1:1000.
Gould R.A., Aziz H., Woods C.E., Seman-Senderos M.A., Sparks E., Preuss C., Wünnemann F., Bedja D., Moats C.R., McClymont S.A., Rose R., Sobeira N., Ling H., MacCarrick G., Anand Kumar A., Luyckx I., Cannaerts E., Verstraeten A., Björk H.M., Lehsau A.C., Jaskula-Ranga V., Lauridsen H., Shah A.A., Bennett C.L., Ellinor P.T., Lin H., Isselbacher E.M., Cardenas C.L., Butcher J.T., Hughes G.C., Lindsay M.E., Mertens L., Franco-Cereceda A., Verhagen J.M., Wessels M., Mohamed S.A., Per E., Mital S., Laer L.V., Loeys B.L., Andelfinger G., McCallion A.S, & Dietz H.C. (2018). ROBO4 Variants Predispose Individuals to Bicuspid Aortic Valve and Thoracic Aortic Aneurysm. Nature genetics, 51(1), 42-50.
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+ Expand

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