Hrp labeled anti rabbit or anti mouse igg secondary antibodies

Western blots were performed using standard protocols: protein lysates were resolved in 10% SDS-PAGE and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA). The primary antibodies and dilutions used were the following: rabbit polyclonal anti-NIS (1:500; 24324-1-AP, Proteintech, Rosemont, IL, USA); rabbit polyclonal anti-GFP (1:10,000; ab290, Abcam, Cambridge, UK); rabbit poly-clonal anti-ERK 1/2 antibody (1:1000; #4695, Cell Signaling, Danvers, MA, USA); mouse monoclonal anti-HA (1:10,000; 11 583 816 001, Roche, Mannheim, Germany); mouse monoclonal anti-phospho-ERK anti-body (1:1000; M8159, Sigma-Aldrich, St. Louis, MO, USA); mouse monoclonal anti-PCNA (1:2000; NA03, Merck, Darmstadt, Germany); mouse monoclonal anti-RAC1 (1:1000; 05-389, Millipore, Burlington, MA, USA) and mouse monoclonal anti-alpha tubulin (1:300,000; T5168, Sigma-Aldrich, St. Louis, MO, USA). HRP-labeled anti-rabbit or anti-mouse IgG secondary antibodies (1:3000; Bio-Rad, Hercules, CA, USA) were used for chemiluminescence imaging.

Western blots were performed using standard protocols in 10% SDS-PAGE and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA). Primary antibody and dilutions used were the following: mouse monoclonal anti-HA (11 583 816 001, Roche, Mannheim, Germany); rabbit polyclonal anti-NIS (24324-1-AP, Protein-tech, Rosemont, IL, USA); mouse monoclonal anti-PCNA (NA03, Merck, Darmstadt, Germany); rabbit anti-GLUT1 (ab652; Abcam, Cambridge, UK); goat anti-GLUT1 (sc-1603; Santa Cruz Biotechnology, Santa Cruz, CA, USA); mouse monoclonal anti-RAC1 (05-389, Millipore, Burlington, MA, USA); mouse monoclonal anti-SRC antibody (60315-1-lg, Proteintech, Rosemont, IL, USA); rabbit anti-phospho Y416-SRC antibody D49G4 (#6943, Cell Signaling, Danvers, MA, USA); mouse monoclonal anti-VAV2 antibody (sc-271442, Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit polyclonal anti-phospho Y172-VAV2 antibody (ab86695, Abcam, Cambridge, UK); mouse monoclonal anti-P120 catenin antibody 15D2 (#33-9600, Thermofisher, Waltham, MA, USA); rabbit polyclonal anti-phospho Y228-P120 catenin (329050; US Biological, Salem, MA, USA). HRP-labeled anti-rabbit or anti-mouse IgG secondary antibodies (Bio-Rad, Hercules, CA, USA) were used for chemiluminescence imaging.